Abstract: Disclosed herein are methods and materials for isolating and identifying T cell receptors from single cells. In some embodiments, genomic DNA from a single T cell is isolated using whole genome amplification (WGA). A series of PCR reactions is carried out to enrich the genomic template for sequences encoding the TCR alpha and beta chains, and then to isolate the sequences encoding the TCR alpha and beta chains.

Abstract: A route manager queries network elements to identify egress routes and external nodes coupled to the network elements. The route manager queries a database to identify telephone numbers associated with the external nodes. For each telephone number associated with an external node, the route manager associates the telephone number with the egress routes coupled to the external node and with the network elements coupled to the egress routes. On a call, the route manager selects one of the network elements and the egress routes associated with the telephone number.

Abstract: A visor for shielding the interior of a vehicle from the light or glare from a light source includes a visor that can be rotated about an axis from a stowed position to a shielding position. The visor may also be translated along a track so that the placement of the visor along the length of a large window may be chosen with precision.

Abstract: A seat for a vehicle and more particularly a front seat for a vehicle includes a central pillar extending from the floor to the roof of the vehicle, and a center support extending forwardly from the central pillar. A lower rib extends laterally from the center support and supports a seat bottom fixed thereto. Primary and secondary intermediate ribs also extend laterally from the central pillar. A seat back is fixed to and supported by the primary and secondary intermediate ribs. Also, an upper rib is provided which extends laterally from the central pillar above the intermediate ribs. A headrest is fixed to and supported by the upper rib.

Abstract: A thread structure for an optical lens comprises a plastic lens barrel, and the thread structure is formed on an outer surface of the lens barrel. In each single circle of the thread structure there is a normal thread and a partial withdraw thread. The partial withdraw thread is located on a longitudinal parting line of the thread structure, a top diameter and a pitch circle diameter of the partial withdraw thread are smaller than those of the normal thread, the thread structure is provided with ribs that are located at the root of a first normal thread close to the front end of the lens barrel. The withdraw thread produces an interval between the thread structure and the base, thus preventing the burrs on the parting surface from contacting the inner threads of the base and causing powder.

Abstract: A thread structure for an optical lens comprises a plastic lens barrel, and the thread structure is formed on an outer surface of the lens barrel. In each single circle of the thread structure there is a normal thread and a partial withdraw thread. The partial withdraw thread is located on a longitudinal parting line of the thread structure, a top diameter and a pitch circle diameter of the partial withdraw thread are smaller than those of the normal thread, the thread structure is provided with ribs that are located at the root of a first normal thread close to the front end of the lens barrel. The withdraw thread produces an interval between the thread structure and the base, thus preventing the burrs on the parting surface from contacting the inner threads of the base and causing powder.