Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.

Abstract: A vessel for transporting a material that is solid or semi-solid at ambient temperature, includes a body having an interior surface comprising textured metal, and a superoleophobic coating on the interior surface for inhibiting the material from adhering to the interior surface, the superoleophobic coating including a nanotextured coating disposed on the textured metal and functionalized with a fluorinated compound. The superoleophobic coating facilitates flow of the material along the interior surface.

Abstract: Architecture that facilitates management of a semiconductor and/or microelectronics manufacturing process. A manufacturing component in communication with the semiconductor and/or microelectronics process operates according to process conditions to output a product, which manufacturing component at least one of monitors and controls the process using modularized code. A rules engine component processes one or more rules in association with the modularized code to control the process conditions in realtime by balancing process efficiency criteria to arrive at an optimal result.