Abstract: The present disclosure provides a system and methods for time of flight imaging, comprising: (a) an imaging sensor configured to receive a plurality of light signals reflected from a surgical scene, wherein the imaging module comprises a first imaging unit configured for time of flight (TOF) imaging; a second imaging unit configured for at least one of laser speckle imaging and fluorescence imaging; and an optical element configured to (i) direct a first set of light signals to the first imaging unit and (ii) direct a second set of light signals to the second imaging unit; and (b) an image processing module operatively coupled to the first imaging unit and the second imaging unit, wherein the image processing module is configured to generate one or more images of the surgical scene based on the first set of light signals and the second set of light signals.