Patents by Inventor Michael W. Lassner
Michael W. Lassner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10435699Abstract: Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.Type: GrantFiled: January 24, 2013Date of Patent: October 8, 2019Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Saverio Carl Falco, Michael W Lassner, Zhongsen Li, Christopher Jay Scelonge
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Publication number: 20180298397Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: March 23, 2018Publication date: October 18, 2018Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL W. LASSNER, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK A. LYZNIK, DAVID J. PETERSON, CHRISTOPHER JAY SCELONGE
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Publication number: 20180298396Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: March 23, 2018Publication date: October 18, 2018Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL W. LASSNER, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK A. LYZNIK, DAVID J. PETERSON, CHRISTOPHER JAY SCELONGE
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Patent number: 9951346Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: GrantFiled: July 18, 2012Date of Patent: April 24, 2018Assignees: Pioneer Hi-Bred International, Inc., E. I. du Pont de Nemours and CompanyInventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael W. Lassner, Zhongsen Li, Zhan-Bin Liu, Leszek A. Lyznik, David J. Peterson, Christopher Jay Scelonge
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Publication number: 20140338070Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: July 18, 2012Publication date: November 13, 2014Applicants: PIONEER HI-BRED INTERNATIONAL, E.I. DU PONT DE NEMOURS AND COMPANYInventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael W. Lassner, Zhongsen Li, Zhan-Bin Liu, Leszek A. Lyznik, David J. Peterson, Christopher Jay Scelonge
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Publication number: 20140075597Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.Type: ApplicationFiled: September 6, 2013Publication date: March 13, 2014Inventors: Marc C Albertsen, Howard P. Hershey, Michael W. Lassner, Yongzhong Wu
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Publication number: 20140033371Abstract: Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.Type: ApplicationFiled: October 3, 2013Publication date: January 30, 2014Applicants: PIONEER HI-BRED INTERNATIONAL INC, E I DU PONT DE NEMOURS AND COMPANYInventors: KEVIN E. MCBRIDE, MICHAEL W. LASSNER, LOREN L. LOOGER, BRIAN MCGONIGLE
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Patent number: 8580556Abstract: Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.Type: GrantFiled: August 1, 2012Date of Patent: November 12, 2013Assignees: E. I. du Pont de Nemours and Company, Pioneer Hi-Bred International Inc.Inventors: Kevin E McBride, Michael W Lassner, Loren L Looger, Brian McGonigle
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Publication number: 20110008868Abstract: Multizymes (i.e., single polypeptides having at least two independent and separable enzymatic activities) are disclosed for a variety of applications beyond the synthesis of long chain polyunsaturated fatty acids.Type: ApplicationFiled: April 3, 2009Publication date: January 13, 2011Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: Howard Glenn Damude, Steven Gutteridge, Anthony J. Kinney, Michael W. Lassner, Daniel L. Siehl
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Patent number: 7709666Abstract: Provided are recombinant constructs comprising DNA sequences encoding enzymes effective in altering the biosynthesis and accumulation of sterol compounds and tocopherols in transgenic plants. Also provided are methods of using such constructs to produce transgenic plants, seeds of which contain elevated levels of sitostanol and/or sitostanol esters, and ?-tocopherol, as well as reduced levels of campesterol and campestanol and their corresponding esters. These seeds also contain the novel sterol brassicastanol. Oil obtained from seeds of such transgenic plants is also provided. This oil can be used to prepare food and pharmaceutical compositions effective in lowering the level of low density lipoprotein cholesterol in blood serum. In addition, novel DNA sequences encoding plant steroid 5?-reductases are also disclosed.Type: GrantFiled: January 16, 2004Date of Patent: May 4, 2010Assignee: Monsanto Technology LLCInventors: Mylavarapu Venkatramesh, David R. Corbin, B. Ganesh Bhat, Sekhar S. Boddupalli, Robert J. Grebenok, Ganesh M. Kishore, Kathryn D. Lardizabal, Michael W. Lassner, Shaukat H. Rangwala, Balasulojini Karunanandaa
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Patent number: 7332311Abstract: By this invention, nucleic acid sequences encoding for fatty acyl-CoA:fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl-CoA substrates. Of special interest are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33 kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.Type: GrantFiled: April 18, 2003Date of Patent: February 19, 2008Assignee: Calgene LLCInventors: Kathryn D. Lardizabal, James G. Metz, Michael W. Lassner
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Patent number: 7265207Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: GrantFiled: January 23, 2003Date of Patent: September 4, 2007Assignee: Calgene LLCInventors: Michael W. Lassner, Beth Savidge, Timothy A. Mitsky, James D. Weiss, Martha Ann Post-Beittenmiller
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Patent number: 7141718Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: GrantFiled: May 13, 2003Date of Patent: November 28, 2006Assignee: Calgene LLCInventors: Michael W. Lassner, Timothy Mitsky, James Weiss, Martha Ann Post-Beittenmiller
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Patent number: 7041872Abstract: By this invention, novel nucleic acid sequences encoding for acyl-CoA: cholesterol acyltransferase (ACAT) related proteins are provided, wherein said ACAT-like protein is active in the formation of a sterol ester and/or triacylylgycerol from a fatty acyl-CoA and sterol and/or diacylglycerol substrates. Also considered are amino acid and nucleic acid sequences obtainable from ACAT-like nucleic acid sequences and the use of such sequences to provide transgenic host cells capable of producing sterol esters and/or triacylglycerols.Type: GrantFiled: May 31, 2002Date of Patent: May 9, 2006Assignee: Calgene LLCInventors: Michael W. Lassner, Diane M. Ruezinsky
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Patent number: 6828475Abstract: By this invention, a plant &bgr;-ketoacyl-CoA synthase condensing enzyme is provided free from intact cells of said plant and capable of catalyzing the production of very long chain fatty acid molecules. Also contemplated are constructs comprising the nucleic acid sequence and a heterologous DNA sequence not naturally associated with the condensing enzyme encoding sequences, and which provide for at least transcription of a plant condensing enzyme encoding sequence in a host cell. In this fashion very long chain fatty acid molecules may be produced in a plant cell. Included are methods of modifying the composition of very long chain fatty acid molecules in a plant cell.Type: GrantFiled: May 30, 1996Date of Patent: December 7, 2004Assignee: Calgene LLCInventors: James George Metz, Kathryn Dennis Lardizabal, Michael W. Lassner
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Publication number: 20040018602Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: ApplicationFiled: May 13, 2003Publication date: January 29, 2004Inventors: Michael W. Lassner, Beth Savidge, Timothy A. Mitsky, James D. Weiss, Martha Ann Post-Beittenmiller
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Publication number: 20030228668Abstract: By this invention, nucleic acid sequences encoding for fatty acyl-CoA:fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl-CoA substrates. Of special interest is are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33 kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.Type: ApplicationFiled: April 18, 2003Publication date: December 11, 2003Inventors: Kathryn Dennis Lardizabal, James George Metz, Michael W. Lassner
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Publication number: 20030170833Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: ApplicationFiled: January 23, 2003Publication date: September 11, 2003Inventors: Michael W. Lassner, Beth Savidge, Timothy Mitsky, James D. Weiss, Martha Ann Post-Beittenmiller
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Patent number: 6596538Abstract: By this invention, nucleic acid sequences encoding for fatty acyl-CoA: fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl-CoA substrates. Of special interest is are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33 kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.Type: GrantFiled: June 5, 1998Date of Patent: July 22, 2003Assignee: Calgene LLCInventors: Kathryn Dennis Lardizabal, James George Metz, Michael W. Lassner
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Patent number: 6541259Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: GrantFiled: April 14, 2000Date of Patent: April 1, 2003Assignee: Calgene LLCInventors: Michael W. Lassner, Beth Savidge, Timothy Mitsky, James Weiss, Martha Ann Post-Beittenmiller