Patents by Inventor Michael W. Pantoliano

Michael W. Pantoliano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, asparate 101 or leucine 137
    Patent number: 7138374
    Abstract: The present invention relates to novel muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for stimulating cell division, treating a wound, treating ischemia, treating heart disease, treating neural injury, treating peripheral vascular disease, treating a gastric ulcer and treating a duodenal ulcer.
    Type: Grant
    Filed: February 23, 2004
    Date of Patent: November 21, 2006
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Barry A. Springer, Michael W. Pantoliano, Celia M. Sharp
  • High throughput method for functionally classifying proteins identified using a genomics approach
    Patent number: 7122321
    Abstract: The present invention provides a method for functionally classifying a protein that is capable of unfolding due to a thermal change. The method comprises screening one or more of a multiplicity of different molecules for their ability to shift the thermal unfolding curve of the protein, wherein a shift in the thermal unfolding curve indicates that the molecule binds to the protein or affects the stability in a measurable way; generating an activity spectrum for the protein wherein the activity spectrum reflects a set of molecules, from the multiplicity of molecules, that shift the thermal unfolding curve, of the protein and therefore are ligands that bind to the protein, comparing the activity spectrum for the protein to one or more functional reference spectrum lists; and classifying the protein according to the set of molecules in the multiplicity of different molecules that shift the thermal unfolding curve of the protein.
    Type: Grant
    Filed: January 29, 2002
    Date of Patent: October 17, 2006
    Assignee: Johnson & Johnson Pharmaceutical Research & Development, L.L.C.
    Inventors: Michael W Pantoliano, Francis R Salemme, Theodore E Carver, Jr.
  • Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
    Patent number: 6849458
    Abstract: The present invention provides an assay apparatus for that includes a temperature adjusting means for simultaneously heating a plurality of samples, and a receiving means for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving means can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving means can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting means can be configured with a temperature controller for changing temperature in accordance with a predetermined profile.
    Type: Grant
    Filed: March 9, 2001
    Date of Patent: February 1, 2005
    Inventors: Michael W. Pantoliano, Roger F. Bone, Alexander W. Rhind, Francis R. Salemme
  • Crystal structure of angiotensin-converting enzyme-related carboxypeptidase
    Publication number: 20040209344
    Abstract: The invention relates to molecules or molecular complexes which comprise binding pockets of angiotensin-converting enzyme-related carboxypeptidase or its homologues. The invention relates to a computer comprising a data storage medium encoded with the structure coordinates of such binding pockets. The invention also relates to methods of using the structure coordinates to solve the structure of homologous proteins or protein complexes. The invention relates to methods of using the structure coordinates to screen for and design compounds that bind to angiotensin-converting enzyme-related carboxypeptidase protein or homologues thereof. The invention also relates to crystallizable compositions and crystals comprising angiotensin-converting enzyme-related carboxypeptidase protein or angiotensin-converting enzyme-related carboxypeptidase protein complexes.
    Type: Application
    Filed: September 9, 2003
    Publication date: October 21, 2004
    Inventors: Michael W. Pantoliano, M. Dominic Ryan, Bart Lee Staker, G. Sridhar Prasad, Jin Tang, Saurabh Prabhakar Menon, Paul S. Towler, David H. Williams, Martin Fisher
  • Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
    Publication number: 20040185504
    Abstract: The present invention provides an assay apparatus for that includes a temperature adjusting means for simultaneously heating a plurality of samples, and a receiving means for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving means can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving means can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting means can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.
    Type: Application
    Filed: April 7, 2004
    Publication date: September 23, 2004
    Inventors: Michael W. Pantoliano, Roger F. Bone, Alexander W. Rhind, Francis R. Salemme
  • Analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, Aspartate 101 or Leucine 137
    Patent number: 6767722
    Abstract: The present invention relates to muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed is a method for stimulating cell division.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: July 27, 2004
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Barry A. Springer, Michael W. Pantoliano, Celia M. Sharp
  • Methods of using analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamate 89, aspartate 101 or leucine 137
    Patent number: 6737404
    Abstract: The present invention relates to novel muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for stimulating cell division, treating a wound, treating ischemia, treating heart disease, treating neural injury, treating peripheral vascular disease, treating a gastric ulcer and treating a duodenal ulcer.
    Type: Grant
    Filed: April 5, 2001
    Date of Patent: May 18, 2004
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Barry A. Springer, Michael W. Pantoliano, Celia M. Sharp
  • Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
    Publication number: 20030203497
    Abstract: The present invention provides an assay apparatus for that includes a temperature adjusting means for simultaneously heating a plurality of samples, and a receiving means for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving means can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving means can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting means can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.
    Type: Application
    Filed: April 22, 2003
    Publication date: October 30, 2003
    Inventors: Michael W. Pantoliano, Roger F. Bone, Alexander W. Rhind, Francis R. Salemme
  • Microplate thermal shift assay for ligand development using 5- (4"-dimethylaminophenyl) -2- (4' -phenyl) oxazole derivative fluorescent dyes
    Publication number: 20030175813
    Abstract: The present invention provides a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of unfolding due to a thermal change.
    Type: Application
    Filed: February 14, 2003
    Publication date: September 18, 2003
    Applicant: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, F. Raymond Salemme, Barry A. Springer
  • Microplate thermal shift assay for ligand development using 5-(4?dimethylaminophenyl)-2-(4?-phenyl)oxazole derivative fluorescent dyes
    Patent number: 6569631
    Abstract: The present invention provides a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of unfolding due to a thermal change.
    Type: Grant
    Filed: November 12, 1999
    Date of Patent: May 27, 2003
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, F. Raymond Salemme, Barry A. Springer
  • High throughput method for functionally classifying proteins identified using a genomics approach
    Publication number: 20020168686
    Abstract: The present invention provides a method for functionally classifying a protein that is capable of unfolding due to a thermal change. The method comprises screening one or more of a multiplicity of different molecules for their ability to shift the thermal unfolding curve of the protein, wherein a shift in the thermal unfolding curve indicates that the molecule binds to the protein or affects the stability in a measurable way; generating an activity spectrum for the protein wherein the activity spectrum reflects a set of molecules, from the multiplicity of molecules, that shift the thermal unfolding curve, of the protein and therefore are ligands that bind to the protein, comparing the activity spectrum for the protein to one or more functional reference spectrum lists; and classifying the protein according to the set of molecules in the multiplicity of different molecules that shift the thermal unfolding curve of the protein.
    Type: Application
    Filed: January 29, 2002
    Publication date: November 14, 2002
    Inventors: Michael W. Pantoliano, F. Raymond Salemme, Theodore E. Carver
  • Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
    Publication number: 20020114734
    Abstract: The present invention provides an assay apparatus for that includes a temperature adjusting means for simultaneously heating a plurality of samples, and a receiving means for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving means can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving means can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting means can be configured with a temperature controller for changing temperature in accordance with a predetermined profile.
    Type: Application
    Filed: March 9, 2001
    Publication date: August 22, 2002
    Inventors: Michael W. Pantoliano, Roger F. Bone, Alexander W. Rhind, Francis R. Salemme
  • Method for identifying lead compounds
    Patent number: 6303322
    Abstract: The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change.
    Type: Grant
    Filed: January 5, 2000
    Date of Patent: October 16, 2001
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Alexander W. Rhind, Francis R. Salemme
  • Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization
    Patent number: 6291191
    Abstract: The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change.
    Type: Grant
    Filed: December 2, 1999
    Date of Patent: September 18, 2001
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, Alexander W. Rhind, Francis R. Salemme, Barry A. Springer
  • Method for identifying conditions that facilitate recombinant protein folding
    Patent number: 6291192
    Abstract: The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change.
    Type: Grant
    Filed: January 5, 2000
    Date of Patent: September 18, 2001
    Assignee: 3-Dimensional Pharmaceuticals Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, Francis R. Salemme, Barry A. Springer
  • Analogs of human basic fibroblast growth factor
    Publication number: 20010020004
    Abstract: The present invention relates to novel muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of said nucleic acid sequences and the production of said muteins. Also disclosed are methods for stimulating cell division, treating a wound, treating ischemia, treating heart disease, treating neural injury, treating peripheral vascular disease, treating a gastric ulcer and treating a duodenal ulcer.
    Type: Application
    Filed: April 5, 2001
    Publication date: September 6, 2001
    Applicant: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Barry A. Springer, Michael W. Pantoliano, Celia M. Sharp
  • Analogs of human basic fibroblast growth factor mutated at one or more of the positions glutamute 89, aspartate 101 or leucine 137
    Patent number: 6274712
    Abstract: The present invention relates to muteins of human basic fibroblast growth factor with superagonist properties. Both protein and the respective encoding nucleic acid species are disclosed. The invention also embodies vectors and host cells for the propagation of the nucleic acid sequences and the production of the muteins.
    Type: Grant
    Filed: December 23, 1998
    Date of Patent: August 14, 2001
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Barry A. Springer, Michael W. Pantoliano, Celia M. Sharp
  • Method for determining conditions that facilitate protein crystallization
    Patent number: 6268158
    Abstract: The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change.
    Type: Grant
    Filed: December 10, 1999
    Date of Patent: July 31, 2001
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, Francis R. Salemme, Barry A. Springer
  • Method for sensing and processing fluorescence data from multiple samples
    Patent number: 6268218
    Abstract: The present invention provides an assay apparatus for that includes a temperature adjusting device for simultaneously heating a plurality of samples, and a receiving device for receiving spectral emission from the samples while the samples are being heated. In further aspects of the invention, the receiving device can be configured to receive fluorescent emission, ultraviolet light, and visible light. The receiving device can be configured to receive spectral emission from the samples in a variety of ways, e.g., one sample at a time, simultaneously from more than one sample, or simultaneously from all of the samples. The temperature adjusting device can be configured with a temperature controller for changing temperature in accordance with a pre-determined profile.
    Type: Grant
    Filed: December 14, 1999
    Date of Patent: July 31, 2001
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Roger F. Bone, Alexander W. Rhind, Francis R. Salemme
  • HIGH THROUGHPUT METHOD FOR FUNCTIONALLY CLASSIFYING PROTEINS IDENTIFIED USING A GENOMICS APPROACH
    Publication number: 20010003648
    Abstract: The present invention provides a method for functionally classifying a protein that is capable of unfolding due to a thermal change. The method comprises screening one or more of a multiplicity of different molecules for their ability to shift the thermal unfolding curve of the protein, wherein a shift in the thermal unfolding curve indicates that the molecule binds to the protein or affects the stability in a measurable way; generating an activity spectrum for the protein wherein the activity spectrum reflects a set of molecules, from the multiplicity of molecules, that shift the thermal unfolding curve, of the protein and therefore are ligands that bind to the protein, comparing the activity spectrum for the protein to one or more functional reference spectrum lists; and classifying the protein according to the set of molecules in the multiplicity of different molecules that shift the thermal unfolding curve of the protein.
    Type: Application
    Filed: November 12, 1998
    Publication date: June 14, 2001
    Inventors: MICHAEL W. PANTOLIANO, F. RAYMOND SALEMME, THEODORE E. CARVER