Abstract: Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

Abstract: An inverter for the selective feeding of effective power and reactive power into a power grid has two serially connected intermediate circuit capacitors, the joint connection of which defines a median voltage level between a positive voltage level and a negative voltage level. It also has first, second, third, and fourth semiconductor switches having free-wheeling diodes respectively connected in parallel, which are arranged serially in this sequence between the positive and the negative voltage levels, the joint connection of the second and third semiconductor switches being connected to the power grid via a choke. The inverter also has serially connected first and second diodes, the joint connection of which is at the median voltage level, and whose second connection is connected to the joint connection of the first and second semiconductor switches and of the third and fourth semiconductor switches.

Abstract: An inverter for the selective feeding of effective power and reactive power into a power grid has two serially connected intermediate circuit capacitors, the joint connection of which defines a median voltage level between a positive voltage level and a negative voltage level. It also has first, second, third, and fourth semiconductor switches having free-wheeling diodes respectively connected in parallel, which are arranged serially in this sequence between the positive and the negative voltage levels, the joint connection of the second and third semiconductor switches being connected to the power grid via a choke. The inverter also has serially connected first and second diodes, the joint connection of which is at the median voltage level, and whose second connection is connected to the joint connection of the first and second semiconductor switches and of the third and fourth semiconductor switches.

Abstract: A method and apparatus for a pressurized gas cylinder transportation system, which may be rapidly loaded, unloaded and refueled. A transportation system may include transportation equipment, a lift system, a cylinder holding cage, a single cylinder hand truck and a gas refueling station to deliver the cylinders.