Patents by Inventor Michaela M. Sieben

Michaela M. Sieben has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for the synthesis of tryptophan analogs in aqueous solvents at reduced temperatures
    Patent number: 10752927
    Abstract: The present disclosure provides methods for preparing tryptophans and tryptophan derivatives. The methods include: combining i) an indole substrate or azulene substrate, ii) a serine substrate, and iii) an engineered tryptophan synthase ?-subunit (TrpB); and maintaining the resulting mixture under conditions sufficient to form the product compound. The engineered TrpB comprises a PLP binding loop mutation, a helix 1 mutation, a strand 7-8 mutation, or a combination thereof. New TrpB variants are also described.
    Type: Grant
    Filed: March 1, 2019
    Date of Patent: August 25, 2020
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Christina E. Boville, David K. Romney, Patrick J. Almhjell, Michaela M. Sieben
  • METHOD FOR THE SYNTHESIS OF TRYPTOPHAN ANALOGS IN AQUEOUS SOLVENTS AT REDUCED TEMPERATURES
    Publication number: 20190271016
    Abstract: The present disclosure provides methods for preparing tryptophans and tryptophan derivatives. The methods include: combining i) an indole substrate or azulene substrate, ii) a serine substrate, and iii) an engineered tryptophan synthase ?-subunit (TrpB); and maintaining the resulting mixture under conditions sufficient to form the product compound. The engineered TrpB comprises a PLP binding loop mutation, a helix 1 mutation, a strand 7-8 mutation, or a combination thereof. New TrpB variants are also described.
    Type: Application
    Filed: March 1, 2019
    Publication date: September 5, 2019
    Applicant: California Institute of Technology
    Inventors: Christina E. Boville, David K. Romney, Patrick J. Almhjell, Michaela M. Sieben