Abstract: The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a m

Abstract: The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a m

Abstract: The present invention relates to a method for detecting analytes in a sample comprising the steps of providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are also capable of binding to the analytes, thus concentrating the analytes on the solid support, providing detection probes which are capable of binding to the analytes, contacting the sample with the detection probes, the solid support and the capture probes, and detecting through use of confocal observation the analytes which are bound to the detection probes.

Abstract: The present invention relates to a method for detecting analytes in a sample comprising the steps of providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are also capable of binding to the analytes, thus concentrating the analytes on the solid support, providing detection probes which are capable of binding to the analytes, contacting the sample with the detection probes, the solid support and the capture probes, and detecting through use of confocal observation the analytes which are bound to the detection probes.

Abstract: The invention relates to a method for investigating the modulating effect of a test substance on a PKC?-dependent signal transduction pathway or for finding a PKC? modulator in a human or animal cell, including the steps (a) contacting the cell with the test substance or with the PKC? modulator; (b) where appropriate inducing the kinase activity of PKC?; (c) incubating the cell under conditions which bring about phosphorylation at least of a serine or threonine residue of PKC?; (d) where appropriate lysing the cell; and (e) determining the phosphorylation content of the at least one serine or threonine residue of PKC?.