Abstract: A method and device for standardizing myoblast differentiation into myotubes, including a substrate (1) and at least one cell-adhesive pattern (2) for culturing myoblasts on the substrate. The pattern (2) has an elongated surface. A central region (2C) and two lateral regions (2L) extend from the central region in both directions along a longitudinal axis of the pattern. The ratio between the maximum width (WC) of the central region (2C) and the maximum width (WL) of the lateral regions (2L) is greater than or equal to 2. The ratio between the length (L) and the maximum width (WC) of the pattern (2) is less than or equal to 4. The method includes providing a device as described above, depositing myoblasts on at least one cell-adhesive pattern of the device, culturing the myoblasts in a differentiation medium to promote cell differentiation into myotubes and constrain elongation of the myotubes.

Abstract: The invention relates to a device for standardizing myoblast differentiation into myotubes, comprising a substrate (1) and at least one cell-adhesive pattern (2) for culturing myoblasts on said substrate, wherein: said pattern (2) has an elongated surface comprising a central region (2C) and two lateral regions (2L) extending from said central region in both directions along a longitudinal axis of the pattern with a contour discontinuity between the central region (2C) and each lateral region (2L), the ratio between the maximum width (WC) of the central region (2C) and the maximum width (WL) of the lateral regions (2L) is greater than or equal to 2, the ratio between the length (L) and the maximum width (WC) of the pattern (2) is less than or equal to 4.

Abstract: The present invention relates to devices and associated methods for forming three-dimensional multicellular assemblies in vitro. Specifically, the present invention relates to devices comprising at least one three-dimensional multicellular assembly immobilised on a two-dimensional adhesive pattern, wherein said three-dimensional multicellular assembly has an organised structure with a normalised polarity and methods for the formation of three-dimensional multicellular assemblies having an organised structure.

Abstract: The present invention relates to methods and devices for adhering cells in a specific and predetermined position with an adhesive control of internal cell organization, methods for preparing such devices, methods for studying modifications of cell shape and global internal cell organization such as the distribution of cellular compartments, centrosome centering, spindle orientation, internal compartmentalization and internal transports, methods for screening compounds of interest which enhance or inhibit specific cell functions.

Abstract: The present invention relates to methods and devices for adhering cells in a specific and predetermined position with an adhesive control of internal cell organisation, methods for preparing such devices, methods for studying modifications of cell shape and global internal cell organization such as the distribution of cellular compartments, centrosome centering, spindle orientation, internal compartimentalization and internal transports, methods for screening compounds of interest which enhance or inhibit specific cell functions.