Abstract: The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.

Abstract: The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.

Abstract: The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.

Abstract: The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens.

Abstract: An anti-theft locking device for vehicles includes an annular locking sleeve mounted concentrically about and normally in frictional engagement with the vehicle steering shaft. The locking sleeve includes a body portion having a serpentine cross-sectional configuration, thereby defining on the inner periphery of the sleeve a plurality of alternate smooth convex projections and concave valleys. The convex projections are in frictional engagement with the circumferential surface of the steering shaft by a predetermined frictional force that is greater than the rotational torque force produced during normal steering operation of the vehicle. A plurality of circumferentially spaced teeth extend radially outwardly from the locking sleeve, thereby to define between the teeth a plurality of locking slots one of which is arranged to receive the free extremity of the locking arm of a locking device.

Abstract: An anti-theft locking device for vehicles includes an annular locking sleeve mounted concentrically about and normally in frictional engagement with the vehicle steering shaft. The locking sleeve includes a body portion having a serpentine cross-sectional configuration, thereby defining on the inner periphery of the sleeve a plurality of alternate smooth convex projections and concave valleys. The convex projections are in frictional engagement with the circumferential surface of the steering shaft by a predetermined frictional force that is greater than the rotational torque force produced during normal steering operation of the vehicle. A plurality of circumferentially spaced teeth extend radially outwardly from the locking sleeve, thereby to define between the teeth a plurality of locking slots one of which is arranged to receive the free extremity of the locking arm of a locking device.

Abstract: This invention relates to crystalline pharmaceutically acceptable salts of an alpha 1a adrenergic receptor antagonist, Compound A, which are useful in the treatment of benign prostatic hyperplasia. Pharmaceutical compositions employing the crystalline salts, and processes for making and using the crystalline salts and pharmaceutical compositions of Compound A are also disclosed. This invention further relates to a process for obtaining enantiomerically pure intermediate useful for the synthesis of end product alpha 1a adrenergic receptor antagonists. The end product compounds are useful for the treatment of benign prostatic hyperplasia and for relaxing lower urinary tract tissue. The invention also relates to a process for preparing a class of dihydropyrimidinone compounds of which Compound A is a member, wherein the process involves deprotonating a dihydropyrimidinone compound and then coupling the deprotonated derivative with a primary amine.

Abstract: In a device for holding a first member in position relative to a second member, the first member and the second member are connected by a clamping system having a clamping axle passing through the members. A relatively soft plastics material adhesion shoe is fixed to the second member. The clamping axle passes through the adhesion shoe. A metal rack having teeth is disposed on a base which is mounted on the clamping axle. When the clamping system is immobilized in a locked position, the teeth of the rack penetrate the relatively soft plastics material of the adhesion shoe in order to guarantee that the first member is held in position relative to the second member.

Abstract: A bioconversion process where benzyl acetoacetate is converted to benzyl-(S)-(+)-3-hydroxybutyrate.

Abstract: The present invention is concerned with a novel process for the preparation of a compound of the formula: ##STR1## This compound is useful as an intermediate in the synthesis of compounds which possess pharmacological activity.

Abstract: In a method of producing splines on a shaft, cold deformation without removal of chips is effected on the outside face of a small-thickness tube in a single operation. The cold deformation includes hammering the small-thickness tube by a conjugate and simultaneous action of beating and kneading. The depth of the splines can be as much as half the thickness of the tube.

Abstract: A method for preparing (1S)-amino-(2R)-indandiol substantially free of its stereoisomers is presented. The method comprises culturing indene with Rhodococcus sp. ATCC 55805, recovering the indandiol and subjecting the indandiol to chiral specific crystallization, treatment with strong acid and reverse ion pair extraction.

Abstract: A biotransformation process for the production of an intermediate used in the chemical synthesis of an antibiotic compound.