Abstract: The invention concerns a method for determining at least one target molecule map in a tissue section, using at east one (A-X)n-B conjugate, wherein A is a tag molecule of known molecular weight, X is a linker that is cleaved during sample desorption/ionization, n is an integer of at least 1, and B is a binding molecule that binds specifically to said target molecule. When using MALDI mass spectrometry, said linker molecule X may be cleaved by photodissociation during sample laser irradiation if photocleavable at the wavelength of said MALDI laser. Alternatively, when using UV-MALDI, IR-MALDI, SIMS or DESI mass spectrometry, said linker molecule X may be cleaved by fragmentation during sample desorption/ionization.

Abstract: Forming process, during vulcanization on a mandrel, of the end portions of rubber tubes, using a tool having a radially deformable skirt in the form of a corolla, made of vulcanized rubber which encloses the end portions of rubber tubes using solely the action of the circumferential elasticity of its corolla. The disengagement of the part after vulcanization is accomplished by a simple axial thrust on the corolla. An upturned position of the radially deformable skirt makes possible the installation of the rubber tubes and other preparation operations.