Abstract: The invention concerns a method for preparing nucleic acids from an environment sample, more particularly a method for obtaining a library of nucleic acids from a sample. The invention also concerns nucleic acids of nucleic acid libraries obtained by said method their use in the synthesis of novel compounds, in particular novel compounds of therapeutic interest. The invent further concerns novel means used in the method for obtaining said nucleic acids, such as novel vectors and novel processes for preparing such vectors or recombinant host cells containing said nucleic acid. Finally, the invention concerns methods for detecting a nucleic acid of interest within a library of nucleic acids resulting from said method, and nucleic acids detected by said method and polypeptides encoded by said nucleic acids.

Abstract: Novel nucleic acid sequences, vectors for expressing same, and uses of said sequences, in particular in actinomycetes fermentation methods.

Abstract: The present invention relates to a vector for cloning and expression of a DNA sequence coding for a particular protein in an Actynomycetes strain. The process comprises:an attachment sequence att,a DNA sequence coding for an int sequnce functional in said strain, anda DNA sequence coding for said specific protein and for the elements ensuring the expression of said sequence in said strain.

Abstract: A hybrid plasmid comprising at least the DNA of a bacterial plasmid, all or part of the DNA of the 2.mu. plasmid of yeast and a segment of DNA including the gene URA.sub.3.sup.+ of yeast. These plasmids are useful as vectors of exogenous DNA in yeasts.

Abstract: The invention relates to enzymatic compositions containing intra-cellular glucose-isomerase which is enclosed in structures based on cellulose ester, their preparation and their use for isomerizing glucose into levulose.In addition to the cellulose ester and micro-organism cells, e.g. Streptomyces phaeochromogenes, these compositions contain a sparingly water-soluble magnesium compound and optionally a sparingly water-soluble cobalt compound.These compositions make it possible to isomerize glucose into levulose in a continuous process, with the addition of either no, or a small amount of magnesium ions to the glucose syrup to be isomerized and without the addition of cobalt ions.

Abstract: A process for the enzymatic isomerization of glucose to fructose where the glucose isomerase is combined with magnesium or magnesium and cobalt ions which have been fixed to a cation exchange resin. The glucose isomerase remains combined with the metal ions during the isomerization. The use of the instant complex prolongs the working life of the enzyme and minimizes the need for conventional pH control.