Abstract: To address the limitations deriving from the unspecific genomic cleavages of the Streptococcus pyogenes Cas9 (SpCas9) and to identify variants with higher cleavage fidelity, the present invention describes a yeast-based assay which allows to simultaneously evaluate the on- and off-target activity towards two engineered genomic targets. The screening of SpCas9 variants obtained by random mutagenesis of the Red-II domain allowed the identification of hits with increased on/off ratios. The best performing nuclease, evoCas9, was isolated through the combination of the identified mutations within a single variant. Side by side analyses with previously reported rationally designed variants demonstrated a significant improvement in fidelity of evoCas9 of the present invention.

Abstract: Methods and apparatus to identify lenses of head-wearable apparatus are disclosed. Example glasses disclosed herein include a frame and an image generator coupled to the frame. A first lens is removably carriable by the frame. The first lens has a first identifier to provide a first code representative of a first optical characteristic of the first lens. The image generator is to project an image toward the first lens when the first lens is carried by the frame. A reader is to read the first code when the first lens is carried by the frame.

Abstract: To address the limitations deriving from the unspecific genomic cleavages of the Streptococcus pyogenes Cas9 (SpCas9) and to identify variants with higher cleavage fidelity, the present invention describes a yeast-based assay which allows to simultaneously evaluate the on- and off-target activity towards two engineered genomic targets. The screening of SpCas9 variants obtained by random mutagenesis of the Red-II domain allowed the identification of hits with increased on/off ratios. The best performing nuclease, evoCas9, was isolated through the combination of the identified mutations within a single variant. Side by side analyses with previously reported rationally designed variants demonstrated a significant improvement in fidelity of evoCas9 of the present invention.

Abstract: Methods and apparatus to identify lenses of head-wearable apparatus are disclosed. Example glasses disclosed herein include a frame and an image generator coupled to the frame. A first lens is removably carriable by the frame. The first lens has a first identifier to provide a first code representative of a first optical characteristic of the first lens. The image generator is to project an image toward the first lens when the first lens is carried by the frame. A reader is to read the first code when the first lens is carried by the frame.

Abstract: Electronically reinforced head-wearable apparatus and related methods are disclosed. Example glasses include a frame to carry a first lens. The frame defines a first body. A first stem and a second stem are to couple to the frame. The first stem and the second stem define a second body and a third body, respectively. A circuit board defines a circuit to implement the glasses. The circuit board is shaped to define a framework of at least one of the first body of the frame, the second body of the first stem or the third body of the second stem.