Patents by Inventor Michele Wendel

Michele Wendel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for refolding insoluble aggregates of hepatitis C virus protease
    Patent number: 5714371
    Abstract: A method for solubulizing and refolding insoluble aggregates of HCV protease is presented. Insoluble aggregates of HCV NS3 protease are extracted from bacteria producing the aggregates. The aggregates of HCV NS3 are then solubilized in a buffer containing the denaturing reagent. Solubilized protease is then placed in an acidic buffer containing a reducing agent. The denaturing reagent is then removed from the buffer under acidic conditions. The pH of the buffer containing HCV NS3 protease is then raised in a step-wise manner to a pH of about 7-8 so as to produce properly refolded soluble, active HCV NS3 protease.
    Type: Grant
    Filed: December 13, 1995
    Date of Patent: February 3, 1998
    Assignee: Schering Corporation
    Inventors: Lata Ramanathan, Michele Wendel