Abstract: Recombinant enzymes BesA, BesB, BesC, BesD and/or BesE are used generate non-canonical amino acids comprising a useful functional group, such as an alkynlyl, alkenyl or halogen.

Abstract: Recombinant enzymes BesA, BesB, BesC, BesD and/or BesE are used generate non-canonical amino acids comprising a useful functional group, such as an alkynlyl, alkenyl or halogen.

Abstract: This disclosure provides systems, methods, and apparatus related to artificial photosynthesis. In one aspect a system includes a photoanode chamber including a photoanode assembly, a photocathode chamber including a photocathode assembly, an electrical connection electrically connecting the photoanode assembly and the photocathode assembly, a membrane separating the photoanode chamber and the photocathode chamber, and a microorganism disposed in the photocathode chamber. The photoanode assembly is operable to oxidize water to generate oxygen, protons, and electrons. The membrane is permeable to the protons and operable to allow the protons to travel to the photocathode chamber. The electrical connection provides electrons to the photocathode assembly. The microorganism comprises a metabolic pathway to reduce carbon dioxide and to generate a carbon-based compound using the electrons or hydrogen formed by two protons.

Abstract: The present disclosure relates generally to the production of alcohols, and more specifically to biological platforms for the production of alcohols using monofunctional aldehyde dehydrogenases and monofunctional alcohol dehydrogenases.

Abstract: Organohalides (e.g., organofluorides) represent a rapidly expanding proportion of molecules used in pharmaceuticals, diagnostics, agrochemicals, and materials. The present invention exploits the naturally occurring acetate condensation pathway as a means of introducing halogenated building blocks into natural product scaffolds. In an exemplary embodiment, the invention provides a pathway involving at least one polyketide synthase system and utilizes haloacetate or a haloacetate synthon (e.g., halomalonate) to incorporate an organohalide into the polyketide backbone in vitro. In an exemplary embodiment, the invention provides an analogous method for site-selective introduction of haloacetate or a synthon into polyketide products in vivo. The present invention expands the scope of existing chemical methods for producing halogenated natural product scaffolds such as complex halogenated natural products.

Abstract: This disclosure provides systems, methods, and apparatus related to artificial photosynthesis. In one aspect a system includes a photoanode chamber including a photoanode assembly, a photocathode chamber including a photocathode assembly, an electrical connection electrically connecting the photoanode assembly and the photocathode assembly, a membrane separating the photoanode chamber and the photocathode chamber, and a microorganism disposed in the photocathode chamber. The photoanode assembly is operable to oxidize water to generate oxygen, protons, and electrons. The membrane is permeable to the protons and operable to allow the protons to travel to the photocathode chamber. The electrical connection provides electrons to the photocathode assembly. The microorganism comprises a metabolic pathway to reduce carbon dioxide and to generate a carbon-based compound using the electrons or hydrogen formed by two protons.

Abstract: Organohalides (e.g., organofluorides) represent a rapidly expanding proportion of molecules used in pharmaceuticals, diagnostics, agrochemicals, and materials. The present invention exploits the naturally occurring acetate condensation pathway as a means of introducing halogenated building blocks into natural product scaffolds. In an exemplary embodiment, the invention provides a pathway involving at least one polyketide synthase system and utilizes haloacetate or a haloacetate synthon (e.g., halomalonate) to incorporate an organohalide into the polyketide backbone in vitro. In an exemplary embodiment, the invention provides an analogous method for site-selective introduction of haloacetate or a synthon into polyketide products in vivo. The present invention expands the scope of existing chemical methods for producing halogenated natural product scaffolds such as complex halogenated natural products.

Abstract: The present disclosure provides optimized recombinant cells for the production of n-butanol. Methods for the use of these cells are also provided. Specifically, the utility of acylating aldehyde dehydrogenases and pyruvate:flavodoxin/ferredoxin-oxidoreductase for the improvement of n-butanol yields from recombinant cells is disclosed.