Abstract: A system and method for performing speckle correlation flow cytometry (SCFC). By subtracting out the stationary background when shining light through a sample (e.g., a vessel within a biological tissue), light only scattered by the desired targets (e.g., cells) can be captured and different types of targets (e.g., cells) can be distinguished by the autocorrelation of the speckle pattern. In this way, the targets (e.g., cells) can be classified and counted based on the features of their speckle correlations. The technique can be applied not only for noninvasive, label-free, in vivo CTC counting but also for counting other types of blood cells such as white blood cells or red blood cells.

Abstract: In one embodiment, a sensorless adaptive optics imaging system includes a source of light, an optical delivery unit having a wavefront modifying element, and an optical coherence tomography (OCT) sensor configured to acquire OCT images based on light emitted by the source of light and transmitted through the optical delivery unit. The system also includes a processing unit that can: process the OCT images, and determine an adjustment of parameters of the wavefront modifying element. In some embodiments, the system includes a multi-photon microscopy (MPM) sensor that acquires MPM images based on the light transmitted through the optical delivery unit.

Abstract: A system and method for performing speckle correlation flow cytometry (SCFC). By subtracting out the stationary background when shining light through a sample (e.g., a vessel within a biological tissue), light only scattered by the desired targets (e.g., cells) can be captured and different types of targets (e.g., cells) can be distinguished by the autocorrelation of the speckle pattern. In this way, the targets (e.g., cells) can be classified and counted based on the features of their speckle correlations. The technique can be applied not only for noninvasive, label-free, in vivo CTC counting but also for counting other types of blood cells such as white blood cells or red blood cells.

Abstract: In one embodiment, a sensorless adaptive optics imaging system includes a source of light, an optical delivery unit having a wavefront modifying element, and an optical coherence tomography (OCT) sensor configured to acquire OCT images based on light emitted by the source of light and transmitted through the optical delivery unit. The system also includes a processing unit that can: process the OCT images, and determine an adjustment of parameters of the wavefront modifying element. In some embodiments, the system includes a multi-photon microscopy (MPM) sensor that acquires MPM images based on the light transmitted through the optical delivery unit.