Abstract: Methods of Electrical Discharge Machining (EDM) ceramic components are provided. In one aspect, a method includes electrical discharge machining a ceramic component, such as a Ceramic Matrix Composite (CMC) component. The ceramic component is electrical discharge machined while a contact matrix is positioned so that electrically conductive compliant and pressurized contacts of the contact matrix engage the ceramic component and so that an electrically conductive member of the contact matrix is in electrical conduction to a grounding structure.

Abstract: A system for forming features within composite components includes a tubular electrode extending along a longitudinal direction from a proximal end to a distal end. The distal end is, in turn, configured to be positioned relative to a machining surface of the composite component such that a spark gap is defined between the distal end and the machining surface. Furthermore, the tubular electrode further extends in a radial direction between an inner surface and an outer surface, with the inner surface defining a central passage configured to supply a dielectric fluid to the machining surface. The outer surface of the tubular electrode includes at least one a channel defined therein or a non-circular cross-sectional shape.

Abstract: The present invention provides methods for detecting the presence of a target molecule by the use of nucleotide analogs containing moieties that enable detection. Such analogs may be incorporated into nucleic acids. In one embodiment, nucleotide analogs are used in a process generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators or chain terminators to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase.

Abstract: The present invention provides methods for detecting the presence of a molecule of interest by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a polynucleotide sequence and detecting said products. The invention also provides for methods of multiplex abortive transcription. In a further aspect, the invention provides for the use of dendrimers containing abortive promoter cassettes. In one aspect, the invention provides for abortive promoter cassettes suitable for use in the present invention. In one aspect the products of abortive transcription are detected with the use of mass spectrometry. In another aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription that are detected, including by mass spectrometry.

Abstract: The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, and oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide anologs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.

Abstract: The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.