Abstract: The present disclosure provides compositions and methods for expanding T cells or tumor infiltrating lymphocytes (TILs) in vitro. K562 feeder cells engineered to express a costimulatory molecule (e.g., 41BB ligand (41BBL)) and either interleukin 21 (IL21) or interleukin 7 (IL7) can be used in a rapid expansion protocol (REP) step to expand the T cells or TILs. Thus, provided herein is a culture comprising T-cells or TILs and modified K562 feeder cells. The T cells can be modified to express a chimeric antigen receptor (CAR) or a T cell receptor (TCR) or the TILs can be modified to express membrane-bound IL15 (mbIL15). The T cells or TILs can be expanded in vitro using aREP without the use of exogenous interleukin 2 (IL2), and the expanded cells can be used in adoptive cell therapy for treatment of cancer without concomitant use of an exogenous cytokine such as IL2.

Abstract: A bed toy has doll body that contains a device for indicating when a child should stay in bed or may get up. One or more lights are attached to the doll body and are exteriorly visible. A wireless communication interface contained in the doll body to wirelessly link to a personal access network. An application is executed by a remote user device to transmit one of a time setting, a wake-up time, a light enablement command, over the personal access network to the wireless communication interface. A light driver selectively illuminates the lights to produce change of illumination. A utility executed by a processor determines that a timing event has occurred based upon the time setting and the current time and commands a light driver to change illumination selected from one of no light, a first color and a second color in response to the timing event.

Abstract: A bed toy has doll body that contains a device for indicating when a child should stay in bed or may get up. One or more lights are attached to the doll body and are exteriorly visible. A wireless communication interface contained in the doll body to wirelessly link to a personal access network. An application is executed by a remote user device to transmit one of a time setting, a wake-up time, a light enablement command, over the personal access network to the wireless communication interface. A light driver selectively illuminates the lights to produce change of illumination. A utility executed by a processor determines that a timing event has occurred based upon the time setting and the current time and commands a light driver to change illumination selected from one of no light, a first color and a second color in response to the timing event.

Abstract: A self-heating, self-hydrating pouch to simultaneously heat and hydrate a prepackaged, partially or completely dehydrated food or beverage product that is stored in the pouch. The pouch comprises multiple layers of material that prevent the transmission of water and gasses thereby preventing deterioration of the prepackaged food or beverage product. The pouch includes a membrane filter structure and flameless, exothermic reaction agents. The user adds potable or non-potable water to the pouch. When the water contacts the exothermic reaction agents, an exothermic reaction occurs which heats the water above 200° F. The membrane filter structure filters the heated water to remove bacteria, viruses, chemicals and by-products of the exothermic reaction. The filtered, heated water contacts the partially or completely dehydrated food product or dehydrated beverage product and simultaneously heats and hydrates the food or beverage product.

Abstract: A self-heating, self-hydrating pouch to simultaneously heat and hydrate a prepackaged, partially or completely dehydrated food or beverage product that is stored in the pouch. The pouch comprises multiple layers of material that prevent the transmission of water and gasses thereby preventing deterioration of the prepackaged food or beverage product. The pouch includes a membrane filter structure and flameless, exothermic reaction agents. The user adds potable or non-potable water to the pouch. When the water contacts the exothermic reaction agents, an exothermic reaction occurs which heats the water above 200° F. The membrane filter structure filters the heated water to remove bacteria, viruses, chemicals and by-products of the exothermic reaction. The filtered, heated water contacts the partially or completely dehydrated food product or dehydrated beverage product and simultaneously heats and hydrates the food or beverage product.