Patents by Inventor Michi Egel-Mitani
Michi Egel-Mitani has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8835132Abstract: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ?-amino groups.Type: GrantFiled: August 18, 2009Date of Patent: September 16, 2014Assignee: Novo Nordisk A/SInventors: Ib Jonassen, Michi Egel-Mitani, Per Balschmidt, Jan Markussen, Ivan Diers, Thomas Borglum Kjeldsen
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Publication number: 20100055738Abstract: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ?-amino groups.Type: ApplicationFiled: August 18, 2009Publication date: March 4, 2010Applicant: Novo Nordisk A/SInventors: Ib Jonassen, Michi Egel- Mitani, Per Balschmidt, Jan Markussen, Ivan Diers, Thomas Borglum Kjeldsen
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Patent number: 7595172Abstract: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ?-amino groups.Type: GrantFiled: July 24, 2002Date of Patent: September 29, 2009Assignee: Novo Nordisk A/SInventors: Ib Jonassen, Michi Egel-Mitani, Per Balschmidt, Jan Markussen, Ivan Diers, Thomas Borglum Kjeldsen
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Patent number: 7572884Abstract: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ?-amino groups.Type: GrantFiled: July 28, 2005Date of Patent: August 11, 2009Assignee: Novo Nordisk A/SInventors: Thomas Hoeg-Jensen, Michi Egel-Mitani, Per Balschmidt, Jan Markussen, Ivan Diers
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Publication number: 20050272125Abstract: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ?-amino groups.Type: ApplicationFiled: July 28, 2005Publication date: December 8, 2005Inventors: Thomas Hoeg-Jensen, Michi Egel-Mitani, Per Balschmidt, Jan Markussen, Ivan Diers
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Publication number: 20030144471Abstract: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine &egr;-amino groups.Type: ApplicationFiled: July 24, 2002Publication date: July 31, 2003Inventors: Ib Jonassen, Michi Egel-Mitani, Per Balschmidt, Jan Markussen, Ivan Diers, Thomas Borglum Kjeldsen
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Publication number: 20030082671Abstract: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine &egr;-amino groups.Type: ApplicationFiled: July 24, 2002Publication date: May 1, 2003Inventors: Thomas Hoeg-Jensen, Michi Egel-Mitani, Per Balschmidt, Jan Markussen, Ivan Diers
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Patent number: 6190883Abstract: The invention describes industrial fermentation of a Saccharomyces yeast species for production of a heterologous product encoded by a plasmid or DNA contained in said Saccharomyces yeast species which method utilizes the substrate more efficiently and without fermentative metabolism resulting in formation of ethanol and other unwanted primary products of fermentative activity whereby high yields of the heterologous product are obtained. The Saccharomyces yeast species is preferably a Crabtree negative Saccharomyces species in particular Saccharomyces kluyveri.Type: GrantFiled: September 8, 1999Date of Patent: February 20, 2001Assignee: Novo Nordisk A/SInventors: Aradhana Srivastava, Jure Piskur, Jens Nielsen, Michi Egel-Mitani
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Patent number: 6110703Abstract: The present invention relates to a novel method for the production of short chain polypeptides, including polypeptides having up to 3 disulfide bonds and/or structures rich in basic amino acid residues, and open structured short chain polypeptides, e.g. glucagon, glucagon like peptides and their functional analogues, in genetically modified yeast cells, said genetically modified yeast cells, and a method for the preparation of said yeast cells.Type: GrantFiled: July 7, 1997Date of Patent: August 29, 2000Assignee: Novo Nordisk A/SInventors: Michi Egel-Mitani, Jakob Brandt, Knud Vad
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Patent number: 6074863Abstract: A lipase variant of a parent lipase comprising a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant said amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase. The parent lipase may be a C. antarctica lipase A essentially free from other substances from C. antarctica, which comprises the amino acid sequence shown in SEQ ID No. 2, or a variant of said lipase which (1) has lipase activity, (2) reacts with an antibody reactive with at least one epitope of C. antarctica lipase A having th eamino acid sequence SEQ ID No.Type: GrantFiled: December 22, 1994Date of Patent: June 13, 2000Assignee: Novo Nordisk A/SInventors: Allan Svendsen, Shamkant Anant Pathar, Michi Egel-Mitani, Kim Borch, Ib Groth Clausen, Mogens Trier Hansen
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Patent number: 6020180Abstract: A DNA sequence encoding a lipase having the amino acid sequence shown in SEQ ID NO:2. The lipase has a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant of the amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase.Type: GrantFiled: July 8, 1998Date of Patent: February 1, 2000Assignee: Novo Nordisk A/SInventors: Allan Svendsen, Shamkant Anant Patkar, Michi Egel-Mitani, Kim Borch, Ib Groth Clausen, Mogens Trier Hansen
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Patent number: 5882883Abstract: The invention relates to an improved process for the production of a secondary metabolite comprising (i) fermentation of a microorganism capable of producing said secondary metabolite, and (ii) recovering said metabolite in substantially pure form. Said microorganism has been modified in a manner whereby the expression of one or more of the DNA sequences coding for (a) peptide(s), (a) protein(s) or (an) enzyme(s), involved in or interfering with the biosynthetic pathway of said secondary metabolite, is regulated differently from the regulation of said DNA sequence(s) in the original microorganism. Further contemplated is a process for production of said microorganism, a DNA construct, a vector or transformation vehicle, a microorganism capable of producing secondary metabolite and finally a secondary metabolite product.Type: GrantFiled: March 5, 1997Date of Patent: March 16, 1999Assignee: Novo Nordisk A/SInventors: Michi Egel-Mitani, Henrik M.o slashed.llgaard, Svend Kaasgaard, Klaus Nyegaard Kristiansen