Patents by Inventor Michio Oishi
Michio Oishi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8173778Abstract: A gene encoding a novel protein that works as a guanine nucleotide exchange factor (GEF) for a Rho family protein being one group of small GTP-binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.Type: GrantFiled: December 31, 2009Date of Patent: May 8, 2012Assignee: Daiichi Sankyo Company, LimitedInventors: Osamu Ohara, Takahiro Nagase, Michio Oishi, Hiroshi Yokota, Osamu Kamida
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Publication number: 20100125131Abstract: A gene encoding a novel protein that works as a guanine nucleotide exchange factor (GEF) for a Rho family protein being one group of small GTP-binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.Type: ApplicationFiled: December 31, 2009Publication date: May 20, 2010Applicants: DAIICHI SANKYO COMPANY, LIMITED, KAZUSA DNA RESEARCH INSTITUTE FOUNDATIONInventors: Osamu Ohara, Takahiro Nagase, Michio Oishi, Hiroshi Yokota, Osamu Kamida
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Patent number: 7713700Abstract: An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from Thermus thermophilus, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.Type: GrantFiled: April 3, 2008Date of Patent: May 11, 2010Assignees: Aisin Cosmos R&D Co., Ltd., Riken, Kazusa DNA Research InstituteInventors: Yasushi Shigemori, Takehiko Shibata, Tsutomu Mikawa, Michio Oishi, Osamu Ohara
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Patent number: 7667013Abstract: A gene encoding a novel protein that works as a guanine nucleotide exchange factor (GEF) for a Rho family protein being one group of small GTP-binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.Type: GrantFiled: March 29, 2005Date of Patent: February 23, 2010Assignees: Daiichi Sankyo Company, Limited, Kazusa DNA Research Institute FoundationInventors: Osamu Ohara, Takahiro Nagase, Michio Oishi, Hiroshi Yokota, Osamu Kamida
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Publication number: 20080206775Abstract: An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from Thermus thermophilus, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.Type: ApplicationFiled: April 3, 2008Publication date: August 28, 2008Applicants: AISIN COSMOS R&D CO., LTD., RIKEN, KAZUSA DNA RESEARCH INSTITUTEInventors: Yasushi SHIGEMORI, Takehiko SHIBATA, Tsutomu MIKAWA, Michio OISHI, Osamu OHARA
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Use of KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development
Publication number: 20070128645Abstract: An object of the present invention is to provide use of the KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development. An agent for treating cancer which comprises as an active ingredient a polypeptide encoded by the KIAA0172 gene, a partial sequence thereof or a variant thereof; an agent for treating cancer which comprises as an active ingredient an oligonucleotide including the KIAA0172 gene sequence; an agent for detecting cancer which comprises an antibody which recognizes polypeptide encoded by the KIAA0172 gene; an agent for detecting cancer which comprises an oligonucleotide including the KIAA0172 gene sequence; a composition for treating cancer which comprises said agent for treating cancer and a pharmaceutically acceptable carrier; and a composition for detecting cancer which comprises said agent for detecting cancer and a pharmaceutically acceptable carrier.Type: ApplicationFiled: November 30, 2006Publication date: June 7, 2007Inventors: Ryoichi Kiyama, Keisuke Kitajima, Shinobu Oguchi, Michio Oishi, Osamu Ohara, Takahiro Nagase -
Patent number: 7220548Abstract: The present invention provides a method of constructing a circular DNA library having an increased content of a desired first dsDNA by removing a second dsDNA using RecA protein to introduce a target single strand nucleic acid by homologous recombination at the 3? terminal portion of the second dsDNA, whereby the target DNA has a 3? terminal portion that differs from the 3? terminal portion of the second dsDNA to prevent circularization, thereby creating a triple stranded DNA portion at the 3? terminal end of the second dsDNA, adding Exonuclease I to digest the displaced first strand of the second dsDNA, ligating the DNA fragments to circularize the desired first dsDNA, removing the linear second dsDNA, thereby constructing the circularized DNA library having an increased content of the desired first dsDNA.Type: GrantFiled: March 10, 2004Date of Patent: May 22, 2007Assignees: Aisin Cosmos R&D Co., Ltd., Kazusa DNA Research Institute FoundationInventors: Kazuhiro Kondo, Michio Oishi, Osamu Ohara
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Patent number: 7022501Abstract: The present invention relates to a DNA ligation method. Specifically, a DNA complex comprising a three-stranded structure in each of the ends of double-stranded DNAs, one of which has single-stranded regions at the end, and the other of which does not have single-stranded regions at the end, is formed under the presence of a homologous recombinant protein. Moreover, according to needs, the DNA complex is introduced into host cells, and said cells are cultured.Type: GrantFiled: June 30, 2000Date of Patent: April 4, 2006Assignee: Aisin Seiki Kabushiki KaishaInventors: Yasushi Shigemori, Michio Oishi
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Publication number: 20050260631Abstract: An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from Thermus thermophilus, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.Type: ApplicationFiled: March 17, 2005Publication date: November 24, 2005Applicants: AISIN COSMOS R&D CO., LTD., RIKEN, KAZUSA DNA RESEARCH INSTITUTEInventors: Yasushi Shigemori, Takehiko Shibata, Tsutomu Mikawa, Michio Oishi, Osamu Ohara
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Publication number: 20050202431Abstract: An object of the present invention is to provide use of the KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development. An agent for treating cancer which comprises as an active ingredient a polypeptide encoded by the KIAA0172 gene, a partial sequence thereof or a variant thereof; an agent for treating cancer which comprises as an active ingredient an oligonucleotide including the KIAA0172 gene sequence; an agent for detecting cancer which comprises an antibody which recognizes polypeptide encoded by the KIAA0172 gene; an agent for detecting cancer which comprises an oligonucleotide including the KIAA0172 gene sequence; a composition for treating cancer which comprises said agent for treating cancer and a pharmaceutically acceptable carrier; and a composition for detecting cancer which comprises said agent for detecting cancer and a pharmaceutically acceptable carrier.Type: ApplicationFiled: July 26, 2002Publication date: September 15, 2005Inventors: Ryoichi Kiyama, Keisuke Kitajima, Shinobu Oguchi, Michio Oishi, Osamu Ohara, Takahiro Nagase
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Patent number: 6867001Abstract: An objective of this invention is to provide a method which can specifically enrich a desired DNA with a long insert size from a DNA library and can provide a clone of the DNA directly. This invention provides a method of constructing a DNA library having increased proportion of a first double-stranded DNA therein by removing, from an original library containing the first double-stranded DNA to be increased in proportion, a second double-stranded DNA different from the first double-stranded DNA.Type: GrantFiled: September 24, 2002Date of Patent: March 15, 2005Assignees: Aisin Cosmos R&D Co., Ltd., Kazusa DNA Research Institute FoundationInventors: Kazuhiro Kondo, Michio Oishi, Osamu Ohara
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Patent number: 6849410Abstract: An objective of this invention is to provide a method for detecting DNA polymorphism that has high sensitivity and efficiency and does not need long DNA searching region. A homologous recombination protein RecA makes partial triple strand DNA from target double DNA and oligonucleotide probe complementary to the DNA. The triple strand DNA maintains stable triple strand DNA after RecA protein is removed. The present inventors found that the thermostability of triple strand DNA changes greatly when there is a mismatch between target DNA and oligonucleotide probe because of the existence of polymorphism in the target DNA. Utilizing this change of thermostability, efficient detection of polymorphism in labeled DNA is possible by examining whether oligonucleotide probe is released and the triple strand DNA is solved after heat treatment of triple strand DNA formed using homologous recombination protein.Type: GrantFiled: November 21, 2001Date of Patent: February 1, 2005Assignees: Aisin Cosmos R & D Co., Ltd., Kazusa DNA Research Institute FoundationInventors: Yasushi Shigemori, Michio Oishi, Osamu Ohara
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Publication number: 20040180374Abstract: The present invention provides a method of constructing a circular DNA library having an increased content of a desired nucleic acid by removing a specific DNA from the circular DNA library by using a RecA protein. More specifically, the present invention provides a method of constructing a DNA library having an increased content of the first dsDNA by removing a second dsDNA different from the first dsDNA from a DNA library containing the first dsDNA to be increased in content and the second dsDNA.Type: ApplicationFiled: March 10, 2004Publication date: September 16, 2004Applicants: Aisin Cosmos R&D Co., Ltd., Kazusa DNA Research Institute FoundationInventors: Kazuhiro Kondo, Michio Oishi, Osamu Ohara
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Patent number: 6613522Abstract: The present invention provides a method of constructing a DNA library having increased proportion of a desired nucleic acid(s) therein by removing a nucleic acid(s) other than the desired nucleic acid(s) from a parent library.Type: GrantFiled: June 1, 2001Date of Patent: September 2, 2003Assignees: Aisin Cosmos R&D Co., Ltd., Kazusa DNA Research Institute FoundationInventors: Kazuhiro Kondo, Osamu Ohara, Michio Oishi
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Publication number: 20030064404Abstract: An objective of this invention is to provide a method which can specifically enrich a desired DNA with a long insert size from a DNA library and can provide a clone of the DNA directly. This invention provides a method of constructing a DNA library having increased proportion of a first double-stranded DNA therein by removing, from an original library containing the first double-stranded DNA to be increased in proportion, a second double-stranded DNA different from the first double-stranded DNA.Type: ApplicationFiled: September 24, 2002Publication date: April 3, 2003Applicant: Aisin Cosmos R&D Co., Ltd.Inventors: Kazuhiro Kondo, Michio Oishi, Osamu Ohara
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Patent number: 6541226Abstract: A method for specifically cleaving a double-stranded DNA. The method comprises forming a three-stranded portion comprising the double-stranded DNA portion including the position to be cleaved or its vicinity and an oligonucleotide and treating the three-stranded protion with a nuclease.Type: GrantFiled: April 14, 2000Date of Patent: April 1, 2003Assignee: Aisin Seiki Kabushiki KaishaInventors: Yasushi Shigemori, Michio Oishi
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Publication number: 20030017475Abstract: An objective of this invention is to provide a method for detecting DNA polymorphism that has high sensitivity and efficiency and does not need long DNA searching region.Type: ApplicationFiled: November 21, 2001Publication date: January 23, 2003Inventors: Yasushi Shigemori, Michio Oishi, Osamu Ohara
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Publication number: 20020058268Abstract: The present invention provides a method of constructing a DNA library having increased proportion of a desired nucleic acid(s) therein by removing a nucleic acid(s) other than the desired nucleic acid(s) from a parent library.Type: ApplicationFiled: June 1, 2001Publication date: May 16, 2002Applicant: Aisin Cosmos R&D Co., Ltd.Inventors: Kazuhiro Kondo, Osamu Ohara, Michio Oishi
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Patent number: 5796907Abstract: An optical transmitter having a higher diffusiveness and a higher transmittivity simultaneously is constructed so that a light from a lamp 2 of light source enters an optical fiber materials A and is transmitted to a reflector tube 1 of which mirror surface of the inner side is formed by vacuum deposition. The diffused light components in the reflector tube 1 are reflected on the mirror surface and directed towards an optical fiber materials B and transferred to an exposure device 3 having a PLZT shutter.Type: GrantFiled: November 9, 1995Date of Patent: August 18, 1998Assignee: Noritsu Koki Co., Ltd.Inventors: Teruo Shiba, Michio Oishi, Masazumi Ishikawa, Tohru Tanibata