Abstract: A method of purifying cytidine diphosphate choline, which comprises contacting a cytidine diphosphate choline solution containing a nucleic acid analogue and having a pH of not less than 0.5 and not more than 5.0 with an H-type strongly acidic cation exchange resin, and eluting cytidine diphosphate choline adsorbed onto the resin with water or an aqueous solution having an ion concentration of not more than 0.1 mol/L to separate and purify the cytidine diphosphate choline.

Abstract: A method of purifying cytidine diphosphate choline, which comprises contacting a cytidine diphosphate choline solution containing a nucleic acid analogue and having a pH of not less than 0.5 and not more than 5.0 with an H-type strongly acidic cation exchange resin, and eluting cytidine diphosphate choline adsorbed onto the resin with water or an aqueous solution having an ion concentration of not more than 0.1 mol/L to separate and purify the cytidine diphosphate choline.

Abstract: The present invention provides a polymer comprising a polysaccharide having excellent water absorption properties, moisture absorption properties, moisture retention properties and thickening properties. Said polysaccharide has the following properties: (A) the principal constituents of the sugar composition are rhamnose, fucose, glucose and glucuronic acid which are present in a molar ratio of (1-4):2:(1-8):(1-4); (B) elemental analysis (wt %):C: 36 .+-.3H: 7 .+-.1O: 56 .+-.4,containing 9-13% of crystalline water(C) solubility:slightly soluble in water; soluble in alkalies; insoluble in methanol, ethanol and acetone;(D) UV absorption spectrum:no absorption detected at 280 nm characteristic of proteins (peptides) or at 260 nm characteristic of nucleic acids; and(E) IR absorption spectrum:an absorption pattern characteristic of polysaccharides is observed near 800-1200.sup.-iSaid polysaccharide is produced by a fermentation method using a natural medium or a synthetic medium of Alcaligenes microorganism.

Abstract: The present invention provides a process for producing trans-L-hydroxyproline, which comprises culturing a microorganism which is capable of decomposing amino acids other than trans-L-hydroxyproline but which is substantially incapable of decomposing trans-L-hydroxyproline in a culture medium containing collagen hydrolyzate, and recovering trans-L-hydroxyproline from the resulting culture.The process enhances the content of trans-L-hydroxyproline based on the total weight of amino acids contained in collagen hydrolyzate, and enables efficient production of trans-L-hydroxyproline which is useful as a starting material for the synthesis of medicines.

Abstract: Disclosed is a process for producing crystals of a salt of an acidic amino acid and a basic amino acid, which comprises adding a water-miscible solvent to an aqueous solution of the salt to prepare a solution 90% or more saturated or supersaturated with the salt, heating the resultant solution under reflux until the formation of crystals stops, adding gradually a water-miscible solvent to the solution under reflux to form crystals of the salt, and recovering the crystals from the resultant mixture.

Abstract: A method for purification of mitomycin C comprises;a. a step of treating the culture of a mitomycin C-producing microorganism with reverse phase adsorption resin to adsorb mitomycin C onto the resin;b. a step of eluting mitomycin C with ethyl acetate out of the resin;c. a step of adding phosphate buffer to the eluate and then evaporating ethyl acetate;d. a step of passing, under pressure, the residue remaining after the evaporation through a column packed with a reverse phase adsorbent having a small particle diameter to adsorb mitomycin C onto the resin;e. a step of eluting mitomycin C with aqueous methanol out of the resin under pressure;f. a step of treating the eluate with a reverse phase adsorption resin to adsorb mitomycin C onto the resin;g. a step of eluting mitomycin C with methanol out of the resin; and,h. a step of concentrating the methanol eluate to crystallize mitomycin C.By this method, mitomycin C can be efficiently purified in an industrial scale.

Abstract: KGF-C is obtained by grinding kefir grain, adding water to the ground kefir grain and heating, homogenizing the mixture and centrifuging it, adding ethanol to the obtained supernatant to obtain a precipitate, dissolving the precipitate into water, purifying the obtained liquid and freeze-drying the remaining liquid. The obtained KGF-C is soluble in water and is composed of polysaccharide as principal component and contains a very small amount of nitrogenous component, and, as sugar composition, is composed of glucose and galactose in the ratio 1:1. This KGF-C has strong antitumor action by oral administration.