Abstract: A novel biologically active substance named Islets-Activating Protein obtained from the cultivation of the miocroorganism Bordetella and having insulin secretion promoting action as well as glucose tolerance improving action for mammals.

Abstract: A method of quantative determination of adenosine by means, of competitive immunoassay based on a competitive antigen-antibody reaction. In the competitive antigen-antibody reaction, an antibody is used which is obtained from an animal which has been immunized by introduction thereto of an antigen which comprises a carrier protein bonded with 2'- and 3'-hydroxyls of the adenosine through dicarboxylic acid residues, and a labelled adenosine and 2',3'-diacyladenosine which has been produced by acylation of adenosine in the sample to be assayed or in a standard solution are caused to undergo competitive reaction for the antibody whereby it has been made possible to determine adenosine quantitatively in high sensitivity and in high accuracy.

Abstract: Novel biologically active substances prepared by dissociating the protein obtained from the cultivation of the microorganism Bordetella and having insulin secretion promoting action as well as glucose tolerance improving action for mammals.

Abstract: Process for ultramicro-quantitative determination of acetone comprising (1) reacting radioactive molecular or cationic iodine with the acetone contained in a sample from biological preparations in a strong alkaline solution to produce radioactive iodoform according to a haloform reaction quantitatively, (2) reducing the non-reacted molecular or cationic radioactive iodine to a radioactive iodide, (3) separating the radioactive iodoform from the radioactive iodide, and (4) measuring the radioactivity of the radioactive iodoform by any conventional method. Two types of assay kits suitable for use in this determination of acetone comprise respectively:(A)Reagent 1 = a radioactive iodide;Reagent 2 = an oxidizing agent;Reagent 3 = an alkali agent;Reagent 4 = a reducing agent;Reagent 5 = an acetone standard solution;Reagent 6 = a surface active agent; andReagent 7 = an anion exchanger.

Abstract: An assay kit for assaying adenosine-3',5'-cyclic monophosphate, which is herein referred to as cAMP, guanosine-3',5'-cyclic monophosphate, which is herein referred to as a cGMP, or both; and said assay kit comprising,A container of Reagent 1 comprising succinyl cAMP tyrosine methyl ester; succinyl cGMP tyrosine methyl ester; succinyl cAMP tyrosine methyl ester and succinyl cGMP tyrosine methyl ester in admixture or both succinyl cAMP tyrosine methyl ester and succinyl cGMP tyrosine methyl ester in separate containers, said esters labelled with radioactive iodine;A container of Reagent 2 comprising an anti-cAMP serum, an anti-cGMP serum, an anti-cAMP and anti-cGMP serum in admixture or both anti-cAMP serum and anti-cGMP serum in separate containers;A container of Reagent 3 comprising an imidazole buffer solution of 0.