Abstract: A dental floss holder particularly suited for access to all teeth in the user's mouth and rapid replacement of dental floss. A hollow handle is adapted to hold a source spool of dental floss interior thereto and to receive a plurality of interchangeable bifurcated tips for holding a taut piece of floss at different respective orientations relative to the handle for convenient access to all teeth in the user's mouth. A pivotally mounted tip is also provided for adjustable placement in several convenient orientations. The source end of the dental floss is fixedly grasped by a quickly-operated lock mechanism connected to a shaft interior to the handle which both rotatably supports the spool of floss and transmits rotational or longitudinal motion from an actuator at one end of the handle to the lock mechanism for releasing a fresh piece of dental floss.