Abstract: The present invention relates to a device for detecting an analyte in a sample, of which the sensitivity is improved by changing the structure. The device for detecting analyte in a sample of the present invention has the effect of improving measurement sensitivity and accuracy by arranging a light source and a photosensor to correspond to an inspection area and a control area, forming holes at positions corresponding to respective light source units, the photosensor, the inspection area, and a calibration area, and adding a stick housing having stick housing partitions formed around the holes.