Patents by Inventor Mikhail Belov
Mikhail Belov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12148605Abstract: A method of operating a collision cell (10) in a mass spectrometer is disclosed. The collision cell comprises an entrance aperture (116), an exit aperture (117) and electrodes (113, 114) for producing electric fields. The method comprises feeding ions in a forward axial direction (LD) through the entrance aperture into the collision cell, producing a first electric field to trap ions, and subsequently producing a second electric field to accelerate trapped ions in the forward axial direction. The method further comprises producing a gas flow (G1) which is, at least at the entrance aperture (116) of the collision cell, contrary to the forward axial direction (LD), so as to reduce the kinetic energy of ions in dependence on their collisional cross sections. A collision cell arranged for carrying out the method is also disclosed, as well as a mass spectrometer comprising such a collision cell.Type: GrantFiled: March 26, 2020Date of Patent: November 19, 2024Assignee: Thermo Fisher Scientific (Bremen) GmbHInventors: Mikhail Belov, Lothar Rottman
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Patent number: 11842892Abstract: Ions are injected into an orbital electrostatic trap. An ejection potential is applied to an ion storage device, to cause ions stored in the ion storage device to be ejected towards the orbital electrostatic trap. Synchronous injection potentials are applied to a central electrode of the orbital electrostatic trap and a deflector electrode associated with the orbital electrostatic trap, to cause the ions ejected from the ion storage device to be captured by the electrostatic trap such that they orbit the central electrode. Application of the ejection potential and application of the synchronous injection potentials are each started at respective different times, the difference in times being selected based on desired values of mass-to-charge ratios of ions to be captured by the orbital electrostatic trap.Type: GrantFiled: April 19, 2022Date of Patent: December 12, 2023Assignee: Thermo Fisher Scientific (Bremen) GmbHInventors: Mikhail Belov, Eduard Denisov, Gregor Quiring, Dmitry Grinfeld
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Publication number: 20220238321Abstract: Ions are injected into an orbital electrostatic trap. An ejection potential is applied to an ion storage device, to cause ions stored in the ion storage device to be ejected towards the orbital electrostatic trap. Synchronous injection potentials are applied to a central electrode of the orbital electrostatic trap and a deflector electrode associated with the orbital electrostatic trap, to cause the ions ejected from the ion storage device to be captured by the electrostatic trap such that they orbit the central electrode. Application of the ejection potential and application of the synchronous injection potentials are each started at respective different times, the difference in times being selected based on desired values of mass-to-charge ratios of ions to be captured by the orbital electrostatic trap.Type: ApplicationFiled: April 19, 2022Publication date: July 28, 2022Applicant: Thermo Fisher Scientific (Bremen) GmbHInventors: Mikhail BELOV, Eduard DENISOV, Gregor QUIRING, Dmitry GRINFELD
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Publication number: 20220181130Abstract: A method of operating a collision cell (10) in a mass spectrometer is disclosed. The collision cell comprises an entrance aperture (116), an exit aperture (117) and electrodes (113, 114) for producing electric fields. The method comprises feeding ions in a forward axial direction (LD) through the entrance aperture into the collision cell, producing a first electric field to trap ions, and subsequently producing a second electric field to accelerate trapped ions in the forward axial direction. The method further comprises producing a gas flow (G1) which is, at least at the entrance aperture (116) of the collision cell, contrary to the forward axial direction (LD), so as to reduce the kinetic energy of ions in dependence on their collisional cross sections. A collision cell arranged for carrying out the method is also disclosed, as well as a mass spectrometer comprising such a collision cell.Type: ApplicationFiled: March 26, 2020Publication date: June 9, 2022Inventors: Mikhail Belov, Lothar Rottman
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Patent number: 11328922Abstract: Ions are injected into an orbital electrostatic trap. An ejection potential is applied to an ion storage device, to cause ions stored in the ion storage device to be ejected towards the orbital electrostatic trap. Synchronous injection potentials are applied to a central electrode of the orbital electrostatic trap and a deflector electrode associated with the orbital electrostatic trap, to cause the ions ejected from the ion storage device to be captured by the electrostatic trap such that they orbit the central electrode. Application of the ejection potential and application of the synchronous injection potentials are each started at respective different times, the difference in times being selected based on desired values of mass-to-charge ratios of ions to be captured by the orbital electrostatic trap.Type: GrantFiled: May 22, 2017Date of Patent: May 10, 2022Assignee: Thermo Fisher Scientific (Bremen) GmbHInventors: Mikhail Belov, Eduard Denisov, Gregor Quiring, Dmitry Grinfeld
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Patent number: 9887074Abstract: A method of analyzing macromolecular complex ions, such protein complex ions, by mass spectrometry and apparatus for performing the method, wherein the method comprises: introducing macromolecular complex ions into a first fragmentation device and trapping the complex ions therein for a trapping period; fragmenting the trapped complex ions in the first fragmentation device to produce monomer subunit ions; optionally selecting one or more species of subunit ions by m/z; introducing one or more of the species of subunit ions into a second fragmentation device, spatially separated from the first fragmentation device; fragmenting the subunit ions in the second fragmentation device to produce a plurality of first fragment ions of the subunit ions; and mass analyzing the first fragment ions in a mass analyzer, or subjecting the first fragment ions to one or more further steps of fragmentation to form further fragment ions and mass analyzing the further fragment ions.Type: GrantFiled: May 22, 2015Date of Patent: February 6, 2018Assignee: Thermo Fisher Scientific (Bremen) GmbHInventor: Mikhail Belov
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Publication number: 20170352530Abstract: Ions are injected into an orbital electrostatic trap. An ejection potential is applied to an ion storage device, to cause ions stored in the ion storage device to be ejected towards the orbital electrostatic trap. Synchronous injection potentials are applied to a central electrode of the orbital electrostatic trap and a deflector electrode associated with the orbital electrostatic trap, to cause the ions ejected from the ion storage device to be captured by the electrostatic trap such that they orbit the central electrode. Application of the ejection potential and application of the synchronous injection potentials are each started at respective different times, the difference in times being selected based on desired values of mass-to-charge ratios of ions to be captured by the orbital electrostatic trap.Type: ApplicationFiled: May 22, 2017Publication date: December 7, 2017Inventors: Mikhail BELOV, Eduard DENISOV, Gregor QUIRING, Dmitry GRINFELD
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Publication number: 20150340213Abstract: A method of analyzing macromolecular complex ions, such protein complex ions, by mass spectrometry and apparatus for performing the method, wherein the method comprises: introducing macromolecular complex ions into a first fragmentation device and trapping the complex ions therein for a trapping period; fragmenting the trapped complex ions in the first fragmentation device to produce monomer subunit ions; optionally selecting one or more species of subunit ions by m/z; introducing one or more of the species of subunit ions into a second fragmentation device, spatially separated from the first fragmentation device; fragmenting the subunit ions in the second fragmentation device to produce a plurality of first fragment ions of the subunit ions; and mass analyzing the first fragment ions in a mass analyzer, or subjecting the first fragment ions to one or more further steps of fragmentation to form further fragment ions and mass analyzing the further fragment ions.Type: ApplicationFiled: May 22, 2015Publication date: November 26, 2015Inventor: Mikhail BELOV
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Publication number: 20060006326Abstract: Methods and apparatus for mass filtering based on a-priori biomarker knowledge and elution time intervals for selected ion species from a separation device. A sample may be screened for biomarker patterns based on distinct elutions times for selected ions or peptides. A mass spectrum for species of interest can be tailored by filtering out undesired ions by measuring corresponding elution times and determining a priori selected elution time intervals for desired ion species only. The invention assists in the identification of biomarkers having known mass spectral peaks corresponding to known proteins or ions of interest that are known to elute from a separation device within a pre-defined elution time window.Type: ApplicationFiled: September 14, 2005Publication date: January 12, 2006Inventors: Mikhail Belov, Peter Foley
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Publication number: 20050244973Abstract: The present invention provides methods for diagnosing cancers, such as prostate cancer. Also, methods for evaluating the prostate cancer state of a patient are described herein. These methods involve the detection, analysis, and classification of biological patterns in biological samples. The biological patterns are obtained using, for example, mass spectrometry systems, antibody based techniques, or nucleic acid based techniques. The present invention also includes therapeutic and prophylactic agents that target the biomarkers described herein. Also, the present invention provides methods for the treatment of prostate cancer using the markers described herein or agents that mimic the properties of these markers.Type: ApplicationFiled: May 14, 2004Publication date: November 3, 2005Applicant: Predicant Biosciences, Inc.Inventors: Frank Andel, Hans Bitter, Mikhail Belov, Michael Brown, Alfred Greenquist, Jonathan Heller, Alexander Sassi, John Stults, Kathy Stults
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Patent number: 6958473Abstract: Methods and apparatus for mass filtering based on a-priori biomarker knowledge and elution time intervals for selected ion species from a separation device. A sample may be screened for biomarker patterns based on distinct elutions times for selected ions or peptides. A mass spectrum for species of interest can be tailored by filtering out undesired ions by measuring corresponding elution times and determining a priori selected elution time intervals for desired ion species only. The invention assists in the identification of biomarkers having known mass spectral peaks corresponding to known proteins or ions of interest that are known to elute from a separation device within a pre-defined elution time window.Type: GrantFiled: March 25, 2004Date of Patent: October 25, 2005Assignee: Predicant Biosciences, Inc.Inventors: Mikhail Belov, Peter Foley
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Publication number: 20050211891Abstract: Methods and apparatus for mass filtering based on a-priori biomarker knowledge and elution time intervals for selected ion species from a separation device. A sample may be screened for biomarker patterns based on distinct elutions times for selected ions or peptides. A mass spectrum for species of interest can be tailored by filtering out undesired ions by measuring corresponding elution times and determining a priori selected elution time intervals for desired ion species only. The invention assists in the identification of biomarkers having known mass spectral peaks corresponding to known proteins or ions of interest that are known to elute from a separation device within a pre-defined elution time window.Type: ApplicationFiled: March 25, 2004Publication date: September 29, 2005Applicant: Biospect Inc.Inventors: Mikhail Belov, Peter Foley
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Publication number: 20050133712Abstract: Methods and apparatus for analyzing ions by pipelining data acquisitions with an orthogonal time-of-flight (OTOF) mass spectrometer. A predetermined push sequence is established for launching packets of ions from a source region into a flight tube towards a detection region within the OTOF mass spectrometer such that ions which are launched in adjacent packets do not overlap prior to reaching the detection region. These discrete packets of ions do not intermingle and are launched in accordance with the predetermined push sequence along a propagation path from the source region toward the detection region such that portions of the packets of ions are simultaneously in-flight within the flight tube of the OTOF mass spectrometer. The times of arrival of ions are detected at the detection region to produce time-of-flight scans with signals corresponding to times of arrival for the ions in the launched packets of ions to provide a mass spectrum derived from pipelined data acquisitions.Type: ApplicationFiled: December 17, 2004Publication date: June 23, 2005Applicant: Predicant Biosciences, Inc.Inventors: Mikhail Belov, Peter Foley
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Patent number: 6900431Abstract: A mass spectrometer and associated methods analyze an ion beam by accumulating ions for a sequence of time periods, and driving the accumulated ions in pulses. Differing quantities of ions can be accumulated in the sequential pulses according to a psuedo-random sequence, and the slower ions are overtaken by the faster ions of a subsequent pulse. A mass spectrum may be reconstructed from an overlapping ion detector signal using an inverse of a weighted simplex matrix or inverse Hadamard transform techniques.Type: GrantFiled: March 21, 2003Date of Patent: May 31, 2005Assignee: Predicant Biosciences, Inc.Inventors: Mikhail Belov, Charles A. Fancher, Peter Foley
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Publication number: 20050001163Abstract: A mass spectrometer and associated methods analyze an ion beam by accumulating ions for a sequence of time periods, and driving the accumulated ions in pulses. Differing quantities of ions can be accumulated in the sequential pulses according to a pseudo-random sequence, and the slower ions are overtaken by the faster ions of a subsequent pulse. A mass spectrum may be reconstructed from an overlapping ion detector signal using an inverse of a weighted simplex matrix or inverse Hadamard transform techniques.Type: ApplicationFiled: July 23, 2004Publication date: January 6, 2005Applicant: Biospect, Inc.Inventors: Mikhail Belov, Charles Fancher, Peter Foley
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Publication number: 20040183007Abstract: A mass spectrometer and associated methods analyze an ion beam by accumulating ions for a sequence of time periods, and driving the accumulated ions in pulses. Differing quantities of ions can be accumulated in the sequential pulses according to a pseudo-random sequence, and the slower ions are overtaken by the faster ions of a subsequent pulse. A mass spectrum may be reconstructed from an overlapping ion detector signal using an inverse of a weighted simplex matrix or inverse Hadamard transform techniques.Type: ApplicationFiled: March 21, 2003Publication date: September 23, 2004Applicant: Biospect, Inc.Inventors: Mikhail Belov, Charles A. Fancher, Peter Foley
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Patent number: 6787760Abstract: A method for enhancing the dynamic range of a mass spectrometer by first passing a sample of ions through the mass spectrometer having a quadrupole ion filter, whereupon the intensities of the mass spectrum of the sample are measured. From the mass spectrum, ions within this sample are then identified for subsequent ejection. As further sampling introduces more ions into the mass spectrometer, the appropriate rf voltages are applied to a quadrupole ion filter, thereby selectively ejecting the undesired ions previously identified. In this manner, the desired ions may be collected for longer periods of time in an ion trap, thus allowing better collection and subsequent analysis of the desired ions. The ion trap used for accumulation may be the same ion trap used for mass analysis, in which case the mass analysis is performed directly, or it may be an intermediate trap.Type: GrantFiled: October 12, 2001Date of Patent: September 7, 2004Assignee: Battelle Memorial InstituteInventors: Mikhail Belov, Richard D. Smith, Harold R. Udseth
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Publication number: 20030071206Abstract: A method for enhancing the dynamic range of a mass spectrometer by first passing a sample of ions through the mass spectrometer having a quadrupole ion filter, whereupon the intensities of the mass spectrum of the sample are measured. From the mass spectrum, ions within this sample are then identified for subsequent ejection. As further sampling introduces more ions into the mass spectrometer, the appropriate rf voltages are applied to a quadrupole ion filter, thereby selectively ejecting the undesired ions previously identified. In this manner, the desired ions may be collected for longer periods of time in an ion trap, thus allowing better collection and subsequent analysis of the desired ions. The ion trap used for accumulation may be the same ion trap used for mass analysis, in which case the mass analysis is performed directly, or it may be an intermediate trap.Type: ApplicationFiled: October 12, 2001Publication date: April 17, 2003Inventors: Mikhail Belov, Richard D. Smith, Harold R. Udseth