Patents by Inventor Mikhail SHAPIRO

Mikhail SHAPIRO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12370272
    Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.
    Type: Grant
    Filed: October 14, 2022
    Date of Patent: July 29, 2025
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Anupama Lakshmanan, Arash Farhadi, Suchita P. Nety, Raymond W. Bourdeau, Mikhail Shapiro
  • Publication number: 20250230446
    Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
    Type: Application
    Filed: February 21, 2025
    Publication date: July 17, 2025
    Inventors: Arash FARHADI, Gabrielle H HO, Daniel P SAWYER, Mikhail SHAPIRO, Robert C. HURT, Mengtong DUAN
  • Patent number: 12359208
    Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T-cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and/or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.
    Type: Grant
    Filed: April 15, 2021
    Date of Patent: July 15, 2025
    Assignee: California Institute of Technology
    Inventors: Mohamad H. Abedi, Mikhail Shapiro, Dan I. Piraner, Justin Lee
  • Patent number: 12318315
    Abstract: Methods and systems are provided for decoding movement intentions using functional ultrasound (fUS) imaging of the brain. In one example, decoding movement intentions include determining a memory phase of a cognitive state of the brain, the memory phase between a gaze fixation phase and movement execution phase, and determining one or more movement intentions including one or more of intended effector (e.g., hand, eye) and intended direction (e.g., right, left) according to a machine learning algorithm trained to classify one or more movement intentions simultaneously.
    Type: Grant
    Filed: May 12, 2021
    Date of Patent: June 3, 2025
    Assignees: California Institute of Technology, INSERM (Institut National de la Santé et de la Recherche Médicale), CNRS-CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris
    Inventors: Sumner L Norman, David Maresca, Vasileios Christopoulos, Mikhail Shapiro, Richard A Andersen, Mickael Tanter, Charlie Demene
  • Patent number: 12241069
    Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
    Type: Grant
    Filed: May 15, 2023
    Date of Patent: March 4, 2025
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Arash Farhadi, Gabrielle H Ho, Daniel P Sawyer, Mikhail Shapiro, Robert C. Hurt, Mengtong Duan
  • Patent number: 12109440
    Abstract: The system and process of therapeutic and effective cavitation by using ultrasound to collapse gas vesicles as well as cavitate the bubbles produced from the collapsed gas vesicles. Therapeutic effect includes, but is not limited to lysing cells by cavitation. The cells expressing the gas vesicles can optionally be used as delivery cells to preform tasks such as transporting the gas vesicles into deep tissue areas, releasing compounds at the cavitation site, and more. The gas vesicles can optionally be modified to facilitate getting the bubbles near the cavitation targets by functionalizing the gas vesicles.
    Type: Grant
    Filed: July 29, 2022
    Date of Patent: October 8, 2024
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Avinoam D. Bar-Zion, Atousa Nourmahnad, David Maresca, Mikhail Shapiro
  • Patent number: 11965001
    Abstract: Provided herein are thermomer monomer, thermomer dimers, thermomer monomeric constructs, thermomer dimeric complexes, and related gene expression cassette vectors, cells, surfaces devices, compositions methods and systems, that provide a thermobioswitch suitable to control location and/or binding of cargo moiety of interest in a temperature regulated manner.
    Type: Grant
    Filed: July 13, 2020
    Date of Patent: April 23, 2024
    Assignee: California Institute of Technology
    Inventors: Dan I. Piraner, Mikhail Shapiro
  • Patent number: 11944848
    Abstract: The system and process of therapeutic and effective cavitation by using ultrasound to collapse gas vesicles as well as cavitate the bubbles produced from the collapsed gas vesicles. Therapeutic effect includes, but is not limited to lysing cells by cavitation. The cells expressing the gas vesicles can optionally be used as delivery cells to preform tasks such as transporting the gas vesicles into deep tissue areas, releasing compounds at the cavitation site, and more. The gas vesicles can optionally be modified to facilitate getting the bubbles near the cavitation targets by functionalizing the gas vesicles.
    Type: Grant
    Filed: July 29, 2022
    Date of Patent: April 2, 2024
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Avinoam D. Bar-Zion, Atousa Nourmahnad, David Maresca, Mikhail Shapiro
  • Patent number: 11944423
    Abstract: A method for in-vivo monitoring of a target internal volume of a mammal that includes: (a) placing the target internal volume proximal to a three-dimensional magnetic field generator; (b) generating first, second, and third magnetic field gradients along respective first, second, and third axes that are mutually orthogonal; (c) measuring first, second, and third magnetic fields with a three-dimensional magnetic sensor disposed in an ingestible capsule, the ingestible capsule disposed in the target internal volume; (d) with a controller in electrical communication with the three-dimensional magnetic sensor, generating a magnetic sensor output signal that encodes a measurement of the first, second, and third magnetic fields; (e) broadcasting the magnetic sensor output signal from an antenna disposed in the ingestible capsule, and (f) receiving the magnetic sensor output signal with a receiver. The received magnetic field data can be used to determine the three-dimensional position of the ingestible capsule.
    Type: Grant
    Filed: November 13, 2020
    Date of Patent: April 2, 2024
    Assignee: California Institute of Technology
    Inventors: Saransh Sharma, Mikhail Shapiro, Azita Emami
  • Patent number: 11903595
    Abstract: A three dimensional magnetic sensor attached to a surgical nail is located based on an applied monotonic magnetic field gradient. Another three dimensional magnetic sensor locates a surgical drill. A display generates a real time image of the relative alignment of the surgical drill and of the surgical nail, allowing a surgeon to repair bone fractures.
    Type: Grant
    Filed: July 18, 2022
    Date of Patent: February 20, 2024
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Saransh Sharma, Abhinav Agarwal, Mikhail Shapiro, Azita Emami
  • Publication number: 20230357780
    Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
    Type: Application
    Filed: May 15, 2023
    Publication date: November 9, 2023
    Inventors: Arash FARHADI, Gabrielle H HO, Daniel P SAWYER, Mikhail SHAPIRO, Robert C. HURT, Mengtong DUAN
  • Patent number: 11786218
    Abstract: The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: October 17, 2023
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Daniel P. Sawyer, Arash Farhadi, Avinoam D. Bar-Zion, Mikhail Shapiro
  • Patent number: 11761008
    Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: September 19, 2023
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Arash Farhadi, Gabrielle H Ho, Daniel P Sawyer, Mikhail Shapiro, Robert C. Hurt, Mengtong Duan
  • Publication number: 20230277695
    Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.
    Type: Application
    Filed: October 14, 2022
    Publication date: September 7, 2023
    Inventors: Anupama LAKSHMANAN, Arash FARHADI, Suchita P. NETY, Raymond W. BOURDEAU, Mikhail SHAPIRO
  • Publication number: 20230158171
    Abstract: Provided herein are methods, systems, and related vectors and compositions allowing for noninvasive control of neural circuits. In particular, the methods and systems herein described utilize acoustically targeted chemogenetics to achieve a noninvasive neuromodulation in specifically selected cell-types among spatially selected brain regions.
    Type: Application
    Filed: June 27, 2022
    Publication date: May 25, 2023
    Inventors: Mikhail SHAPIRO, Jerzy O. SZABLOWSKI
  • Patent number: 11653899
    Abstract: The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: May 23, 2023
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Daniel P. Sawyer, Arash Farhadi, Avinoam D. Bar-Zion, Mikhail Shapiro
  • Patent number: 11649462
    Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: May 16, 2023
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Arash Farhadi, Gabrielle H Ho, Daniel P Sawyer, Mikhail Shapiro, Robert C. Hurt, Mengtong Duan
  • Publication number: 20230139561
    Abstract: The system and process of therapeutic and effective cavitation by using ultrasound to collapse gas vesicles as well as cavitate the bubbles produced from the collapsed gas vesicles. Therapeutic effect includes, but is not limited to lysing cells by cavitation. The cells expressing the gas vesicles can optionally be used as delivery cells to preform tasks such as transporting the gas vesicles into deep tissue areas, releasing compounds at the cavitation site, and more. The gas vesicles can optionally be modified to facilitate getting the bubbles near the cavitation targets by functionalizing the gas vesicles.
    Type: Application
    Filed: July 29, 2022
    Publication date: May 4, 2023
    Inventors: Avinoam D. BAR-ZION, Atousa NOURMAHNAD, David MARESCA, Mikhail SHAPIRO
  • Patent number: 11504438
    Abstract: Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: November 22, 2022
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Anupama Lakshmanan, Arash Farhadi, Suchita P. Nety, Raymond W. Bourdeau, Mikhail Shapiro
  • Publication number: 20220346811
    Abstract: A three dimensional magnetic sensor attached to a surgical nail is located based on an applied monotonic magnetic field gradient. Another three dimensional magnetic sensor locates a surgical drill. A display generates a real time image of the relative alignment of the surgical drill and of the surgical nail, allowing a surgeon to repair bone fractures.
    Type: Application
    Filed: July 18, 2022
    Publication date: November 3, 2022
    Inventors: Saransh SHARMA, Abhinav AGARWAL, Mikhail SHAPIRO, Azita EMAMI