Abstract: This disclosure provides an immunogenic composition comprising an amount of isolated dendritic cells comprising at least one fragment of a hepatitis C protein selected from the group consisting of the core protein of hepatitis C and the NS3 protein of hepatitis C, the fragment does not suppress activity of dendritic cells and is effective to produce an immunogenic response against hepatitis C infection and optionally T-lymphocytes activated for a Th1 response. This disclosure also provides a method and a kit for preparing the above composition. Further, this disclosure provides a method for treating or reducing the severity of hepatitis C and a method for enhancing immunoresponse in a patient suffering from hepatitis C virus infection by administering to the patient in need of the treatment the above composition. Finally, this disclose provides a use of the above composition for treating hepatitis C virus infection.