Patents by Inventor Mikiko NAKAMURA

Mikiko NAKAMURA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Agent for improving gene transfer efficiency to mammalian cells
    Patent number: 8633029
    Abstract: Provided is an efficiency improving agent for gene transfer to mammalian cells, a method for improving efficiency of gene transfer to mammalian cells, and a method for transforming mammalian cells. The method is characterized in that tRNA is used in combination with a lipofection reagent. Preferably, the agent may be used so that the tRNA concentration in a lipofection solution falls within the range of 3 to 50 ?g/mL, and the concentration in a culture is approximately 1/10. More preferably, tRNA and PEG may be used in combination with a lipofection reagent. According to the present invention, gene transfer to mammalian cells with high efficiency can be achieved.
    Type: Grant
    Filed: March 13, 2011
    Date of Patent: January 21, 2014
    Assignee: Yamaguchi University
    Inventors: Rinji Akada, Mikiko Nakamura
  • RNF8-FHA DOMAIN-MODIFIED PROTEIN AND METHOD OF PRODUCING THE SAME
    Publication number: 20130143299
    Abstract: Provided is an antigen-binding protein prepared merely by a method of in vitro selection using the RNF8-FHA domain, which has no intramolecular disulfide bond and functions in cells as it is. One to four loops extending from the FHA domain are randomized, and a recognition site for a target molecule is artificially created on the FHA domain surface to construct an RNF8-FHA domain library. Using the library, an antigen-binding protein is efficiently selected in vitro.
    Type: Application
    Filed: May 6, 2011
    Publication date: June 6, 2013
    Applicant: GENEFRONTIER CORPORATION
    Inventors: Kanehisa Kojoh, Shizue Katoh, Akira Miyakoshi, Mikiko Nakamura
  • Agent for Improving Gene Transfer Efficiency to Mammalian Cells
    Publication number: 20130005041
    Abstract: Provided is an efficiency improving agent for gene transfer to mammalian cells, a method for improving efficiency of gene transfer to mammalian cells, and a method for transforming mammalian cells. The method is characterized in that tRNA is used in combination with a lipofection reagent. Preferably, the agent may be used so that the tRNA concentration in a lipofection solution falls within the range of 3 to 50 ?g/mL, and the concentration in a culture is approximately 1/10. More preferably, tRNA and PEG may be used in combination with a lipofection reagent. According to the present invention, gene transfer to mammalian cells with high efficiency can be achieved.
    Type: Application
    Filed: March 13, 2011
    Publication date: January 3, 2013
    Applicant: YAMAGUCHI UNIVERSITY
    Inventors: Rinji Akada, Mikiko Nakamura
  • COMPOSITION FOR SYNTHESIZING PROTEIN WITH REDUCED LIPOPOLYSACCHARIDE CONTAMINATION, METHOD FOR PRODUCING PROTEIN USING SAID COMPOSITION
    Publication number: 20120231496
    Abstract: According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.
    Type: Application
    Filed: May 3, 2011
    Publication date: September 13, 2012
    Applicants: THE UNIVERSITY OF TOKYO, GENEFRONTIER CORPORATION
    Inventors: Takashi KANAMORI, Yuki HAYAMI, Kanehisa KOJOH, Takuya UEDA, Kumiko TSUIHIJI, Tomoe FUSE, Mikiko NAKAMURA, Shizue KATO