Abstract: There are provided methods and systems for detecting and/or quantifying an analyte. In particular, there are provided methods and systems for simultaneous detection and/or quantitation of two or more analytes in a sample. In some embodiments, there are provided colocalization-by-linkage assays on microparticles (CLAMP) comprising two sets of binders pre-assembled on a support, such that the two sets of binders are colocalized before contacting the sample.

Abstract: Provided herein are colocalization-by-linkage assay (CLA) compositions and methods for multiplexed analysis of an analyte or analytes. The CLA compositions and methods, as described herein, are engineered to detect multiple analytes using multiple detection reagents coupled to a single support. Readout or detection sensitivity is achieved through the use of release-dependent transduction (RDT) or displacer-dependent transduction. Further multiplexing can also be achieved through the use of barcoded elements used in the capture and detection of an analytes.

Abstract: There are provided methods and systems for detecting and/or quantifying an analyte. In particular, there are provided methods and systems for simultaneous detection and/or quantitation of two or more analytes in a sample. Colocalization-by-linkage assays on microparticles (CLAMP) can be engineered and used to effectively multiplex the detection of analytes within a sample. Features and methods of CLAMP systems can provide robust and scalable analysis of analytes in a sample.

Abstract: There are provided methods and systems for detecting and/or quantifying an analyte. In particular, there are provided methods and systems for simultaneous detection and/or quantitation of two or more analytes in a sample. In some embodiments, there are provided colocalization-by-linkage assays on microparticles (CLAMP) comprising two sets of binders pre-assembled on a support, such that the two sets of binders are colocalized before contacting the sample.

Abstract: Provided herein are colocalization-by-linkage assay (CLA) compositions and methods for multiplexed analysis of an analyte or analytes. The CLA compositions and methods, as described herein, are engineered to detect multiple analytes using multiple detection reagents coupled to a single support. Readout or detection sensitivity is achieved through the use of release-dependent transduction (RDT) or displacer-dependent transduction. Further multiplexing can also be achieved through the use of barcoded elements used in the capture and detection of an analytes.

Abstract: There are provided methods and systems for detecting and/or quantifying an analyte. In particular, there are provided methods and systems for simultaneous detection and/or quantitation of two or more analytes in a sample. In some embodiments, there are provided colocalization-by-linkage assays on microparticles (CLAMP) comprising two sets of binders pre-assembled on a support, such that the two sets of binders are colocalized before contacting the sample.

Abstract: Systems and methods for optimizing detection of light-emissive components of a multi-fluorescence spectra. The method comprises obtaining a multi-fluorescence based spectra of a plurality of light-emissive components and determining a model of ensemble multi-fluorescence of said light-emissive components that are stochastically distributed.