Abstract: A disposable allergy skin testing kit that is formed from a top layer sheet, a membrane sheet, and a bottom layer sheet. The bottom sheet has a plurality of recesses formed at predetermined locations to form chambers into which a predetermined antigen has been deposited. The membrane sheet covers these chambers and forms a liquid tight seal. The top layer sheet has an aperture formed in it above each of the antigen chambers. A pushbutton needle assembly is mounted in each of these apertures and it has a disk-shaped pushbutton with a needle extending downwardly from its bottom surface. Flexible support arms attached to the edge of the apertures and the disk-shaped pushbutton allow it to be depressed a sufficient distance in order to rupture the antigen chamber and travel downwardly entirely through it so that the needle penetrates into the patient's skin taking antigen with it.