Patents by Inventor Milan Mrksich
Milan Mrksich has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180080058Abstract: The present disclosure provides methods in which adherent cells are treated with small molecules, cultured, lysed, and then analyzed by mass spectrometry to measure the activities of endogenous enzymes. The implementation of this method relies on the use of surfaces that are nanopatterned with cell adhesion ligands to mediate cell attachment and a peptide that is a substrate for the desired enzyme activity in the lysate.Type: ApplicationFiled: September 20, 2017Publication date: March 22, 2018Inventors: Milan Mrksich, Maria D. Cebezas, Chad A. Mirkin
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Publication number: 20160252501Abstract: Disclosed herein are methods of using an immobilized substrate, immobilized ligand, and a fusion protein of an enzyme for the substrate and a receptor for the ligand, where the immobilized substrate can react to form an immobilized product that has a different mass than the immobilized substrate, and using this transformation to indirectly determine the binding of the receptor and the ligand. These methods can be used for high-throughput screening for possible modulators (e.g., inhibitors or activators) of the ligand-receptor interaction.Type: ApplicationFiled: February 23, 2016Publication date: September 1, 2016Inventors: Milan Mrksich, Patrick O'Kane
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Publication number: 20150369816Abstract: The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.Type: ApplicationFiled: June 26, 2015Publication date: December 24, 2015Inventors: Milan MRKSICH, Christian HODNELAND
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Patent number: 9074300Abstract: The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.Type: GrantFiled: February 4, 2011Date of Patent: July 7, 2015Assignee: The University of ChicagoInventors: Milan Mrksich, Christian Hodneland
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Publication number: 20140256561Abstract: Surface chemistries for the visualization of labeled single molecules (analytes) with improved signal-to-noise properties are provided. To be observed, analyte molecules are bound to surface attachment features that are spaced apart on the surface such that when the analytes are labeled adjacent analytes are optically resolvable from each other. One way to express this concept is that binding elements should be spaced apart such that the Guassian point spread functions of adjacent labels do not overlap. Another way of expressing this concept is that the surface binding elements should be spaced apart by a distance equal to at least the diffraction limit for an optical label attached to the bound analytes.Type: ApplicationFiled: November 27, 2013Publication date: September 11, 2014Applicant: FLUIDIGM CORPORATIONInventors: Jerrod Schwartz, Stephen R. Quake, Milan Mrksich
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Publication number: 20140206570Abstract: The present invention relates to a method of characterizing biochips with matrix-assisted laser desorption/ionization and time of flight mass spectrometry (MALDI-TOF MS).Type: ApplicationFiled: March 25, 2014Publication date: July 24, 2014Applicant: THE UNIVERSITY OF CHICAGOInventors: Milan MRKSICH, Jing SU
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Publication number: 20140134642Abstract: The present invention relates to a method of characterizing biochips with matrix-assisted laser desorption/ionization and time of flight mass spectrometry (MALDI-TOF MS).Type: ApplicationFiled: January 21, 2014Publication date: May 15, 2014Applicant: THE UNIVERSITY OF CHICAGOInventors: Milan MRKSICH, Jing SU
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Publication number: 20120252730Abstract: Peptides and cyclized analogs thereof that are useful as platelet aggregation inhibitors in the treatment of cardiac disease, including acute coronary syndrome are disclosed.Type: ApplicationFiled: September 2, 2010Publication date: October 4, 2012Applicant: UNIVERSITY OF CHICAGOInventors: Milan Mrksich, Juan Sanchez-Cortes
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Publication number: 20120142547Abstract: The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.Type: ApplicationFiled: February 4, 2011Publication date: June 7, 2012Inventors: Milan Mrksich, Christian Hodneland
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Patent number: 8012382Abstract: Chemically or biochemically active agents or other species are patterned on a substrate surface by providing a micromold having a contoured surface and forming, on a substrate surface, a chemically or biochemically active agent or fluid precursor of a structure. A chemically or biochemically active agent or fluid precursor also can be transferred from indentations in an applicator to a substrate surface. The substrate surface can be planar or non-planar. Fluid precursors of polymeric structures, inorganic ceramics and salts, and the like can be used to form patterned polymeric articles, inorganic salts and ceramics, reactive ion etch masks, etc. at the surface. The articles can be formed in a pattern including a portion having a lateral dimension of less than about 1 millimeter or smaller. The indentation pattern of the applicator can be used to transfer separate, distinct chemically or biochemically active agents or fluid precursors to separate, isolated regions of a substrate surface.Type: GrantFiled: March 4, 2009Date of Patent: September 6, 2011Assignee: President and Fellows of Harvard CollegeInventors: Enoch Kim, Younan Xia, Milan Mrksich, Rebecca J. Jackman, Xiao-Mei Zhao, Stephen P. Smith, Christian Marzolin, Mara G. Prentiss, George M. Whitesides
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Patent number: 7888055Abstract: A substrate comprises a surface, and a plurality of moieties, on at least a portion of the surface. The moieties are moieties of formula: Surf-L-Q-T, where -T comprises a reactant ligand, and Surf- designates where the moiety attaches to the surface. The substrate can be made into a protein chip by the reaction of a reactant ligand and a fusion polypeptide, where the fusion polypeptide includes a capture polypeptide moiety which corresponds to the reactant ligand.Type: GrantFiled: December 21, 2006Date of Patent: February 15, 2011Assignee: The University of ChicagoInventors: Milan Mrksich, Christian Hodneland
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Patent number: 7875197Abstract: Improved methods of forming a patterned self-assembled monolayer on a surface and derivative articles are provided. According to one method, an elastomeric stamp is deformed during and/or prior to using the stamp to print a self-assembled molecular monolayer on a surface. According to another method, during monolayer printing the surface is contacted with a liquid that is immiscible with the molecular monolayer-forming species to effect controlled reactive spreading of the monolayer on the surface. Methods of printing self-assembled molecular monolayers on nonplanar surfaces and derivative articles are provided, as are methods of etching surfaces patterned with self-assembled monolayers, including methods of etching silicon. Optical elements including flexible diffraction gratings, mirrors, and lenses are provided, as are methods for forming optical devices and other articles using lithographic molding.Type: GrantFiled: August 6, 2008Date of Patent: January 25, 2011Assignee: President and Fellows of Harvard CollegeInventors: George M. Whitesides, Younan Xia, James L. Wilbur, Rebecca J. Jackman, Enoch Kim, Mara G. Prentiss, Milan Mrksich, Amit Kumar, Christopher B. Gorman, Hans Biebuyck, Karl K. Berggren
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Publication number: 20100112722Abstract: The present disclosure relates to methods of characterizing protein-protein interaction or conducting immunoassays in a biological sample using self-assembled monolayers (SAMs) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (SAMDI). The biological sample may be obtained from a living subject, such as a human or animal clinical sample containing multiple unknown proteins and/or antigens. Label-free methods of identifying protein-protein interactions, antigen-antibody binding and/or diagnosing a medical condition based on analysis of a biological sample using SAMDI are also provided, as well as biochips comprising surface bound proteins and/or antibodies and methods of making these biochips.Type: ApplicationFiled: September 23, 2009Publication date: May 6, 2010Inventors: Milan Mrksich, Violeta L. Marin, Steven M. Patrie
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Publication number: 20100004137Abstract: The present invention relates to a method of characterizing biochips with matrix-assisted laser desorption/ionization and time of flight mass spectrometry (MALDI-TOF MS).Type: ApplicationFiled: January 4, 2005Publication date: January 7, 2010Inventors: Milan Mrksich, Jing Su
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Publication number: 20090166903Abstract: Chemically or biochemically active agents or other species are patterned on a substrate surface by providing a micromold having a contoured surface and forming, on a substrate surface, a chemically or biochemically active agent or fluid precursor of a structure. A chemically or biochemically active agent or fluid precursor also can be transferred from indentations in an applicator to a substrate surface. The substrate surface can be planar or non-planar. Fluid precursors of polymeric structures, inorganic ceramics and salts, and the like can be used to form patterned polymeric articles, inorganic salts and ceramics, reactive ion etch masks, etc. at the surface. The articles can be formed in a pattern including a portion having a lateral dimension of less than about 1 millimeter or smaller. The indentation pattern of the applicator can be used to transfer separate, distinct chemically or biochemically active agents or fluid precursors to separate, isolated regions of a substrate surface.Type: ApplicationFiled: March 4, 2009Publication date: July 2, 2009Applicant: President and Fellows of Harvard CollegeInventors: Enoch Kim, Younan Xia, Milan Mrksich, Rebecca J. Jackman, Xiao-Mei Zhao, Stephen P. Smith, Mara G. Prentiss, George M. Whitesides, Christian Marzolin
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Publication number: 20090053690Abstract: Surface chemistries for the visualization of labeled single molecules (analytes) with improved signal-to-noise properties are provided. To be observed, analyte molecules are bound to surface attachment features that are spaced apart on the surface such that when the analytes are labeled adjacent analytes are optically resolvable from each other. One way to express this concept is that binding elements should be spaced apart such that the Guassian point spread functions of adjacent labels do not overlap. Another way of expressing this concept is that the surface binding elements should be spaced apart by a distance equal to at least the diffraction limit for an optical label attached to the bound analytes.Type: ApplicationFiled: February 1, 2008Publication date: February 26, 2009Applicants: CALIFORNIA INSTITUTE OF TECHNOLOGY, STANFORD UNIVERSITY, UNIVERSITY OF CHICAGOInventors: Jerrod Schwartz, Stephen R. Quake, Milan Mrksich
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Publication number: 20090001049Abstract: Improved methods of forming a patterned self-assembled monolayer on a surface and derivative articles are provided. According to one method, an elastomeric stamp is deformed during and/or prior to using the stamp to print a self-assembled molecular monolayer on a surface. According to another method, during monolayer printing the surface is contacted with a liquid that is immiscible with the molecular monolayer-forming species to effect controlled reactive spreading of the monolayer on the surface. Methods of printing self-assembled molecular monolayers on nonplanar surfaces and derivative articles are provided, as are methods of etching surfaces patterned with self-assembled monolayers, including methods of etching silicon. Optical elements including flexible diffraction gratings, mirrors, and lenses are provided, as are methods for forming optical devices and other articles using lithographic molding.Type: ApplicationFiled: August 6, 2008Publication date: January 1, 2009Applicant: President and Fellows of Harvard CollegeInventors: George M. Whitesides, Younan Xia, James L. Wilbur, Rebecca J. Jackman, Enoch Kim, Mara G. Prentiss, Milan Mrksich, Amit Kumar, Christopher B. Gorman, Hans Biebuyck, Karl K. Berggren
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Publication number: 20080116608Abstract: Chemically or biochemically active agents or other species are patterned on a substrate surface by providing a micromold having a contoured surface and forming, on a substrate surface, a chemically or biochemically active agent or fluid precursor of a structure. A chemically or biochemically active agent or fluid precursor also can be transferred from indentations in an applicator to a substrate surface. The substrate surface can be planar or non-planar. Fluid precursors of polymeric structures, inorganic ceramics and salts, and the like can be used to form patterned polymeric articles, inorganic salts and ceramics, reactive ion etch masks, etc. at the surface. The articles can be formed in a pattern including a portion having a lateral dimension of less than about 1 millimeter or smaller. The indentation pattern of the applicator can be used to transfer separate, distinct chemically or biochemically active agents or fluid precursors to separate, isolated regions of a substrate surface.Type: ApplicationFiled: December 12, 2007Publication date: May 22, 2008Applicant: President and Fellows of Harvard CollegeInventors: Enoch Kim, Younan Xia, Milan Mrksich, Rebecca Jackman, Xiao-Mei Zhao, Stephen Smith, Mara Prentiss, George Whitesides, Christian Marzolin
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Publication number: 20080064606Abstract: A substrate comprises a surface, and a plurality of moieties, on at least a portion of the surface. The moieties are moieties of formula: Surf-L-Q-T, where -T comprises a reactant ligand, and Surf- designates where the moiety attaches to the surface. The substrate can be made into a protein chip by the reaction of a reactant ligand and a fusion polypeptide, where the fusion polypeptide includes a capture polypeptide moiety which corresponds to the reactant ligand.Type: ApplicationFiled: December 21, 2006Publication date: March 13, 2008Inventors: Milan Mrksich, Christian Hodneland
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Publication number: 20070190036Abstract: Microencapsulation of pancreatic islets within a thin poly(ethylene glycol) (PEG) coat is provided. The PEG coat is functionalized with an insulinotropic agent, glucagon-like peptide 1 (GLP-1). The encapsulated pancreatic islets may be used for the treatment of type I diabetes.Type: ApplicationFiled: January 3, 2007Publication date: August 16, 2007Inventors: Seda Kizilel, Jason Wyman, Milan Mrksich, Sidney Nagel, Marc Garfinkel