Patents by Inventor Milenko Despotovic
Milenko Despotovic has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11898196Abstract: The invention includes methods and apparatus for separating mutations, especially rare and unknown mutations, using heteroduplex binding proteins. Nucleic acids may optionally be nicked at or near the mutation in order to promote heteroduplex binding protein recognition and binding. In particular, using the disclosed methods, it is possible to separate heteroduplexed nucleic acid strand pair from homoduplexed nucleic acid strand pairs having similar sequences and being at a much higher concentration. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays.Type: GrantFiled: August 18, 2021Date of Patent: February 13, 2024Assignee: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Matthew Wiggin, Joel Pel
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Publication number: 20240043921Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: ApplicationFiled: October 18, 2023Publication date: February 8, 2024Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali
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Patent number: 11827930Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: GrantFiled: October 1, 2020Date of Patent: November 28, 2023Assignee: NCAN Genomics, Inc.Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali
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Patent number: 11795497Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: GrantFiled: April 9, 2021Date of Patent: October 24, 2023Assignee: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Publication number: 20220056506Abstract: The invention includes methods and apparatus for separating mutations, especially rare and unknown mutations, using heteroduplex binding proteins. Nucleic acids may optionally be nicked at or near the mutation in order to promote heteroduplex binding protein recognition and binding. In particular, using the disclosed methods, it is possible to separate heteroduplexed nucleic acid strand pair from homoduplexed nucleic acid strand pairs having similar sequences and being at a much higher concentration. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays.Type: ApplicationFiled: August 18, 2021Publication date: February 24, 2022Applicant: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Matthew Wiggin, Joel Pel
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Patent number: 11130986Abstract: The invention includes methods and apparatus for separating mutations, especially rare and unknown mutations, using heteroduplex binding proteins. Nucleic acids may optionally be nicked at or near the mutation in order to promote heteroduplex binding protein recognition and binding. In particular, using the disclosed methods, it is possible to separate heteroduplexed nucleic acid strand pair from homoduplexed nucleic acid strand pairs having similar sequences and being at a much higher concentration. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays.Type: GrantFiled: May 19, 2016Date of Patent: September 28, 2021Assignee: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Matthew Wiggin, Joel Pel
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Publication number: 20210238663Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: ApplicationFiled: April 9, 2021Publication date: August 5, 2021Applicant: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Patent number: 10975421Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: GrantFiled: March 13, 2019Date of Patent: April 13, 2021Assignee: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Publication number: 20210087622Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: ApplicationFiled: October 1, 2020Publication date: March 25, 2021Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali
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Patent number: 10829813Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: GrantFiled: June 14, 2018Date of Patent: November 10, 2020Assignee: Boreal Genomics, Inc.Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali
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Patent number: 10738351Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: GrantFiled: April 24, 2019Date of Patent: August 11, 2020Assignee: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Publication number: 20190249234Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: ApplicationFiled: April 24, 2019Publication date: August 15, 2019Applicant: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Publication number: 20190210641Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: ApplicationFiled: March 13, 2019Publication date: July 11, 2019Applicant: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Patent number: 10337054Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: GrantFiled: October 4, 2016Date of Patent: July 2, 2019Assignee: Quantum-Si IncorporatedInventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Publication number: 20190185916Abstract: The invention includes methods and apparatus for separating mutations, especially rare and unknown mutations, using heteroduplex binding proteins. Nucleic acids may optionally be nicked at or near the mutation in order to promote heteroduplex binding protein recognition and binding. In particular, using the disclosed methods, it is possible to separate heteroduplexed nucleic acid strand pair from homoduplexed nucleic acid strand pairs having similar sequences and being at a much higher concentration. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays.Type: ApplicationFiled: May 19, 2016Publication date: June 20, 2019Inventors: Andrea Marziali, Milenko Despotovic, Matthew Wiggin, Joel Pel
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Publication number: 20180298438Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: ApplicationFiled: June 14, 2018Publication date: October 18, 2018Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali
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Patent number: 10000799Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: GrantFiled: November 2, 2015Date of Patent: June 19, 2018Assignee: Boreal Genomics, Inc.Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali
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Publication number: 20170073742Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.Type: ApplicationFiled: October 4, 2016Publication date: March 16, 2017Inventors: Andrea Marziali, Milenko Despotovic, Joel Pel
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Publication number: 20160122814Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: ApplicationFiled: November 2, 2015Publication date: May 5, 2016Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali