Abstract: A way to increase the production of recombinant polypeptides by combining pieces of several different isoforms of a given gene to make a chimeric isoform of the gene (using a chimeric gene can increase the production of polypeptide over 25%), or by inserting several copies of the coding region in tandem into a transformation vector (e.g., plasmids carrying multiple copies of the chimera of SEQ ID NO: 3 increased production 107%).

Abstract: Our invention relates to a new way of preparing coding DNA constructs, by making a chimera of various coding region isoforms. For example, our method has been successfully used to make a new hGH-NV cDNA chimera having SEQ ID NO: 3, by combining the cDNA isoforms of human placenta and pituitary RNA encoding human growth hormone. Our invention also relates to a process for increasing the production of recombinant human growth hormone by inserting a plurality of promoter—coding region—termination sequences into a vector in tandem. For example, our method has been successfully used to approximately double the yield of recombinant growth hormone in transformed cells.