Abstract: A device for viewing a target, the device including a housing, an objective lens positioned within the housing, where the objective lens has a first lens group including at least a first lens and a second lens group including at least a second lens, the first lens positioned closer to the target than the second lens, and a camera positioned within the objective lens between the first lens and the second lens, where the camera is configured to provide images of the target located near a focal point of the objective lens, and wherein the arrangement of the first lens, the second lens, and the camera provides for simultaneous capture of a first image of a surface of the target and a second image of a sub-surface cellular structure of the target.

Abstract: Handheld devices and methods that integrate wide-field dermoscopy with reflectance confocal microscopy for non-invasive simultaneous capture of wide-field color images of the skin surface and images of the sub-surface cellular structure.

Abstract: Provided and described herein are exemplary embodiments of apparatus, system, computer-accessible medium, procedure and method according to the present disclosure which can be used for providing laser steering and focusing for, e.g., incision, excision and/or ablation of tissue in minimally-invasive surgery. For example, an exemplary apparatus is provided that can include at least one optical element which can be configured to refract and/or diffract light provided in a structure which can be configured to be inserted into a body, where at least one of the optical element(s) is structured to receive the light at a first angle and generate a refracted and/or diffracted light at a second angle which can be different from the first angle relative to an optical axis.

Abstract: Provided and described herein are exemplary embodiments of apparatus, system, computer-accessible medium, procedure and method according to the present disclosure which can be used for providing laser steering and focusing for e.g., incision, excision and/or ablation of tissue in minimally-invasive surgery. For example, an exemplary apparatus is provided that can include at least one optical element which can be configured to refract and/or diffract light provided in a structure which can be configured to be inserted into a body, where at least one of the optical element(s) is structured to receive the light at a first angle and generate a refracted and/or diffracted light at a second angle which can be different from the first angle relative to an optical axis.

Abstract: Systems, methods and non-transitory computer-accessible mediums are provided for generating a mosaic image of a large area of tissue with microscopic resolution by acquiring a plurality of rectangular-shaped long strips of images of the tissue and stitching the strips together along their lengths. In some embodiments, systems, methods and non-transitory computer-accessible mediums are provided that can track the position of the translation stage during the scan and/or trigger image acquisition using features patterned on the imaging window, without utilizing an encoder for the stage.

Abstract: Illustrative systems, methods and non-transitory computer-accessible mediums are provided for aligning a focal plane and a sample plane to be imaged.

Abstract: Handheld devices and methods that integrate wide-field dermoscopy with reflectance confocal microscopy for non-invasive simultaneous capture of wide-field color images of the skin surface and images of the sub-surface cellular structure.

Abstract: Exemplary systems, method and non-transitory computer-accessible medium can be provided for generating an image of at least one tissue that can be subdivided into a plurality of strips. Using such exemplary systems, method and computer-accessible medium, it is possible to scan, using an optical arrangement to generate first information, a first portion of the tissue(s) along a first one of the strips while the tissue(s) can be mechanically moved in a first direction. Move the one tissue(s), by a distance equal to or less than a width of the first portion, in a second direction approximately perpendicular to the first direction. Use the optical arrangement to generate second information, a second portion of the tissue(s) along a second one of the strips while the tissue(s) can be mechanically moved in a third direction, which can be approximately opposite to the first direction. Then the image can be generated based on the first information and the second information.

Abstract: Provided and described herein are exemplary embodiments of apparatus, system, computer-accessible medium, procedure and method according to the present disclosure which can be used for providing laser steering and focusing for, e.g., incision, excision and/or ablation of tissue in minimally-invasive surgery. For example, an exemplary apparatus is provided that can include at least one optical element which can be configured to refract and/or diffract light provided in a structure which can be configured to be inserted into a body, where at least one of the optical element(s) is structured to receive the light at a first angle and generate a refracted and/or diffracted light at a second angle which can be different from the first angle relative to an optical axis.

Abstract: Exemplary systems, methods and non-transitory computer-accessible mediums are provided for generating a mosaic image of a large area of tissue with microscopic resolution by acquiring a plurality of rectangular-shaped long strips of images of the tissue and stitching the strips together along their lengths.

Abstract: Exemplary embodiments of apparatus and method for facilitating an analysis of a sample(s) can be provided. For example, using a first arrangement(s), it can be possible to receive the sample(s) thereon. Further, for example, using a second arrangement(s), it can be possible to cause a force to be applied on a portion(s) of the sample(s) such that a surface(s) of the sample(s) can be flattened against a section(s) of the first arrangement(s).

Abstract: A dual-modality apparatus for imaging of biological tissue includes a reflectance confocal microscopy (RCM) imaging apparatus and an optical coherence tomography (OCT) imaging apparatus. A first optical component reflects a first beam of light provided by a RCM imaging apparatus towards a sample and passes a second beam of light provided by an OCT imaging apparatus towards the sample, such that the first and second beam of lights share at least a portion of an imaging path.

Abstract: Provided and described herein are exemplary embodiments of apparatus, system, computer-accessible medium, procedure and method according to the present disclosure which can be used for providing laser steering and focusing for e.g., incision, excision and/or ablation of tissue in minimally-invasive surgery. For example, an exemplary apparatus is provided that can include at least one optical element which can be configured to refract and/or diffract light provided in a structure which can be configured to be inserted into a body, where at least one of the optical element(s) is structured to receive the light at a first angle and generate a refracted and/or diffracted light at a second angle which can be different from the first angle relative to an optical axis.

Abstract: Exemplary embodiments of apparatus, system, computer-accessible medium, procedure and method are provided which can be used for providing laser steering and focusing for, e.g., incision, excision and/or ablation of tissue in minimally-invasive surgery. For example, the exemplary apparatus can include at least one optical element which can be configured to refract and/or diffract light provided in a structure which can be configured to be inserted into a body, where at least one of the optical element(s) is structured to receive the light at a first angle and generate a refracted and/or diffracted light at a second angle which can be different from the first angle relative to an optical axis. For example, an actuating arrangement can be provided, which can be configured to control the optical element(s), can be provided and situated at least partially within the at least one structure.

Abstract: Exemplary embodiments of apparatus and method for facilitating an analysis of a sample(s) can be provided. For example, using a first arrangement(s), it can be possible to receive the sample(s) thereon. Further, for example, using a second arrangement(s), it can be possible to cause a force to be applied on a portion(s) of the sample(s) such that a surface(s) of the sample(s) can be flattened against a section(s) of the first arrangement(s).

Abstract: Exemplary systems, method and non-transitory computer-accessible medium can be provided for generating an image of at least one tissue that can be subdivided into a plurality of strips. Using such exemplary systems, method and computer-accessible medium, it is possible to scan, using an optical arrangement to generate first information, a first portion of the tissue(s) along a first one of the strips while the tissue(s) can be mechanically moved in a first direction. Move the one tissue(s), by a distance equal to or less than a width of the first portion, in a second direction approximately perpendicular to the first direction. Use the optical arrangement to generate second information, a second portion of the tissue(s) along a second one of the strips while the tissue(s) can be mechanically moved in a third direction, which can be approximately opposite to the first direction. Then the image can be generated based on the first information and the second information.

Abstract: Exemplary embodiments of apparatus, system, computer-accessible medium, procedure and method are provided which can be used for providing laser steering and focusing for, e.g., incision, excision and/or ablation of tissue in minimally-invasive surgery. For example, the exemplary apparatus can include at least one optical element which can be configured to refract and/or diffract light provided in a structure which can be configured to be inserted into a body, where at least one of the optical element(s) is structured to receive the light at a first angle and generate a refracted and/or diffracted light at a second angle which can be different from the first angle relative to an optical axis. For example, an actuating arrangement can be provided, which can be configured to control the optical element(s), can be provided and situated at least partially within the at least one structure.

Abstract: A confocal scanning microscope system (10) using cross polarization effects and an enhancement agent (acetic acid) to enhance confocal microscope reflectance images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous cell carcinomas) in the confocal reflectance images of excised tumor slices. The confocal scanning microscope system having a laser (11) for generating an illumination beam (12), a polygon mirror (18) for scanning the beam to a tissue sample (22) and for receiving a returned beam from the tissue sample and detector (28) for detecting the returned beam to form an image. The system further includes a half-wave plate (13) having a rotatable stage (14) and a quarter-wave plate (21) having a rotatable stage (20) disposed in the optical path of the illumination beam and at least a linear polarizer (24) having a rotatable stage (25) disposed in the optical path of the returned beam from the tissue sample.

Abstract: A system using cross polarization effects and an enhancement agent having citric or other similar alpha hydroxy acid to enhance confocal microscope reflectance images and particularly images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous cell carcinomas) in the confocal reflectance images of excised tumor slices obtained during Mohs surgery by illuminating the tissue being imaged (a tumor slice) using polarized light. The reflected illumination is passed to a polarization analyzer, which passes the polarization component which is crossed with respect to the polarization of the illuminating light. The light from the analyzer is passed through the confocal aperture and detected. The section of the tissue either at the surface or within the tissue is scanned and the reflectance image is produced with enhanced visualization of the cellular or nuclear structure thereof thereby enabling determination of the extent of the tumor (cancerous cells) in the section.

Abstract: A confocal scanning microscope system (10) using cross polarization effects and an enhancement agent (acetic acid) to enhance confocal microscope reflectance images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous cell carcinomas) in the confocal reflectance images of excised tumor slices. The confocal scanning microscope system having a laser (11) for generating an illumination beam (12), a polygon mirror (18) for scanning the beam to a tissue sample (22) and for receiving a returned beam from the tissue sample and detector (28) for detecting the returned beam to form an image. The system further includes a half-waveplate (13) having a rotatable stage (14) and a quarter-wave plate (21) having a rotatable stage (20) disposed in the optical path of the illumination beam and at least a linear polarizer (24) having a rotatable stage (25) disposed in the optical path of the returned beam from the tissue sample.