Patents by Inventor Milko B. Kermekchiev
Milko B. Kermekchiev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240026315Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.Type: ApplicationFiled: September 27, 2023Publication date: January 25, 2024Inventors: Wayne M. Barnes, Milko B. Kermekchiev, Zhian Zhang
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Patent number: 11814655Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.Type: GrantFiled: May 28, 2021Date of Patent: November 14, 2023Assignee: DNA POLYMERASE TECHNOLOGY, INC.Inventors: Wayne M. Barnes, Milko B. Kermekchiev, Zhian Zhang
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Publication number: 20230143626Abstract: Amino acid substitutions that provide for increased elongation rates, resistance to PCR inhibitors, and reverse transcriptase activity of Thermus aquaticus (Taq) DNA polymerase enzymes are provided. Also provided are related methods of using the Taq DNA polymerase enzymes to rapidly detect nucleic acids of interest in crude biological samples, without DNA/RNA extraction.Type: ApplicationFiled: October 10, 2022Publication date: May 11, 2023Inventors: Milko B. Kermekchiev, Zhian Zhang
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Publication number: 20210348142Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.Type: ApplicationFiled: May 28, 2021Publication date: November 11, 2021Inventors: Wayne M. Barnes, Milko B. Kermekchiev, Zhian Zhang
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Patent number: 11091745Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.Type: GrantFiled: May 12, 2016Date of Patent: August 17, 2021Assignee: DNA POLYMERASE TECHNOLOGY, INC.Inventors: Wayne M. Barnes, Milko B. Kermekchiev, Zhian Zhang
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Publication number: 20210147908Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.Type: ApplicationFiled: June 15, 2020Publication date: May 20, 2021Inventors: Milko B. Kermekchiev, Zhian Zhang
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Patent number: 10683537Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.Type: GrantFiled: October 16, 2013Date of Patent: June 16, 2020Assignee: DNA Polymerase Technology, Inc.Inventors: Milko B. Kermekchiev, Zhian Zhang
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Publication number: 20180112195Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.Type: ApplicationFiled: May 12, 2016Publication date: April 26, 2018Inventors: Wayne M. Barnes, Milko B. Kermekchiev, Zhian Zhang
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Publication number: 20140113299Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.Type: ApplicationFiled: October 16, 2013Publication date: April 24, 2014Applicant: DNA Polymerase Technology, Inc.Inventors: Milko B. Kermekchiev, Zhian Zhang
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Publication number: 20130252309Abstract: An isolated polypeptide having fast elongating polymerase activity. Also provided are kits containing the isolated polypeptide and isolated polynucleotides encoding the isolated polypeptide.Type: ApplicationFiled: June 1, 2013Publication date: September 26, 2013Applicant: DNA Polymerase Technology Inc.Inventors: Milko B. Kermekchiev, Wayne M. Barnes
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Patent number: 8470563Abstract: A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.Type: GrantFiled: December 8, 2008Date of Patent: June 25, 2013Assignee: DNA Polymerase Technology Inc.Inventors: Milko B. Kermekchiev, Wayne Morris Barnes
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Publication number: 20110027832Abstract: The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.Type: ApplicationFiled: March 16, 2009Publication date: February 3, 2011Applicant: DNA POLYMERASE TECHNOLOGY INC.Inventors: Milko B. Kermekchiev, Lyubka Kirilova, Zhian Zhang
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Publication number: 20090170060Abstract: A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.Type: ApplicationFiled: December 8, 2008Publication date: July 2, 2009Applicant: DNA POLYMERASE TECHNOLOGY INC.Inventors: Milko B. Kermekchiev, Wayne Morris Barnes
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Patent number: 7462475Abstract: A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.Type: GrantFiled: December 6, 2004Date of Patent: December 9, 2008Assignee: DNA Poleymerase Technology, Inc.Inventors: Milko B. Kermekchiev, Wayne M. Barnes
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Publication number: 20020004203Abstract: Provided are mutant DNA polymerases having at least one mutation which exhibit substantially reduced polymerase activity at 25° C. when compared to the same DNA polymerases without the at least one mutation and which exhibit normal or near-normal polymerase activity at optimum temperatures when compared to the same DNA polymerases without the at least one mutation. Also provided are amino acid sequences and nucleic acid sequences encoding such DNA polymerases, and vector plasmids and host cells suitable for the expression of these sequences. Also described herein are improved methods for performing polymerase chain reaction (PCR) amplification and other genetic manipulations and analyses using the mutant DNA polymerases of the invention.Type: ApplicationFiled: February 6, 2001Publication date: January 10, 2002Inventors: Wayne M. Barnes, Milko B. Kermekchiev
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Patent number: 6333159Abstract: Provided are mutant DNA polymerases having at least one mutation which exhibit substantially reduced polymerase activity at 25° C. when compared to the same DNA polymerases without the at least one mutation and which exhibit normal or near-normal polymerase activity at optimum temperatures when compared to the same DNA polymerases without the at least one mutation. Also provided are amino acid sequences and nucleic acid sequences encoding such DNA polymerases, and vector plasmids and host cells suitable for the expression of these sequences. Also described herein are improved methods for performing polymerase chain reaction (PCR) amplification and other genetic manipulations and analyses using the mutant DNA polymerases of the invention.Type: GrantFiled: February 6, 2001Date of Patent: December 25, 2001Assignee: Washington UniversityInventors: Wayne M. Barnes, Milko B. Kermekchiev
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Patent number: 6316202Abstract: Provided are mutant DNA polymerase having at least one mutation which exhibit substantially reduced polymerase activity at 25° C. When compared to the same DNA polymerase without the at least one mutation and which exhibit normal or near-normal polymerase activity at optimum temperatures when compared to the same DNA polymerase without the at least one mutation. Also provided are amino acid sequences and nucleic acid sequences encoding such DNA polymerase, and vector plasmids and host cells suitable for the expression of these sequences. Also described herein are improved methods for performing polymerase chain reaction (PCR) amplification and other genetic manipulations and analyses using the mutant DNA polymerase of the invention.Type: GrantFiled: February 6, 2001Date of Patent: November 13, 2001Assignee: Washington UniversityInventors: Wayne M. Barnes, Milko B. Kermekchiev
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Patent number: 6214557Abstract: Provided are mutant DNA polymerases having at least one mutation which exhibit substantially reduced polymerase activity at 25° C. when compared to the same DNA polymerases without the at least one mutation and which exhibit normal or near-normal polymerase activity at optimum temperatures when compared to the same DNA polymerases without the at least one mutation. Also provided are amino acid sequences and nucleic acid sequences encoding such DNA polymerases, and vector plasmids and host cells suitable for the expression of these sequences. Also described herein are improved methods for performing polymerase chain reaction (PCR) amplification and other genetic manipulations and analyses using the mutant DNA polymerases of the invention.Type: GrantFiled: June 6, 2000Date of Patent: April 10, 2001Assignee: Washington UniversityInventors: Wayne M. Barnes, Milko B. Kermekchiev