Abstract: Immunoassays for measuring thyroid hormones such as thyroxine(T.sub.4) and triiodothyronine(T.sub.3) are carried out by incubating a serum sample with either (a) unlabeled thyroglobulin and a labeled antibody to a thyroid hormone or to thyroxine binding globulin(TBG), or (B) labeled thyrogloublin and an unlabeled antibody to a thyroid hormone. The thyroglobulin may be immobilized on an insoluble carrier and the immobilized thyroglobulin is preferably modified by succinylation with succinic anhydride. Whole thyroglobulin can be used or the thyroglobulin can be fragmented into smaller peptides prior to use provided the peptides contain at least one T.sub.3 or T.sub.4 residue per peptide. Preferably, the antibody is a monocional antibody and labeling is with an acridinium ester.

Abstract: The present invention relates to a novel method for stabilizing aqueous solutions of serum complement for extended periods of time and the resultant stabilized complement solutions. More particularly, morphilino buffering compounds and tris-hydroxymethyl buffering compounds have been used to make aqueous buffers with a pH between about 6.5 and 8.5 and a concentration of greater than 0.01M. These buffers have been found to extend the normal life of complement solutions to unexpectedly longer periods.

Abstract: Glycosylated hemoglobins are separated from other hemoglobin variants by electrochromatography on an agar gel at an acidic pH which utilizes:A. an agar gel having a wet thickness of from about 0.1 to about 0.5 mm.,B. a citrate gel buffer having a citrate concentration of from about 0.02 to about 0.05 M and a pH of from about 5.8 to about 6.8,C. a citrate well buffer having a citrate concentration of from about 0.05 to about 0.1 M and a pH from about 6.0 to about 6.5, andD. a potential of from about 25 to about 100 volts.