Abstract: The present invention provides a method for separating a stromal vascular fraction (SVF) from adipose tissue by using a non-contact ultrasonic device comprising a power supply unit, an ultrasonic oscillation unit, and an ultrasonic vibration unit; and a non-contact ultrasonic device used therefor. Specifically, the present invention provides a method for separating a stromal vascular fraction (SVF) from adipose tissue, and a non-contact ultrasonic device used therefor, in which the stromal vascular fraction can be extracted from adipose tissue with high efficiency while minimizing cell contamination and destruction by using a non-enzymatic method and a non-contact ultrasonic device.

Abstract: A kit for separating adipose tissue-derived stromal vascular fraction is easy to operate so that even a non-specialist can use the kit and can perform washing and suspending processes for stromal vascular fraction in one tube repeatedly at the same time, thereby minimizing exposure to air, resulting in a low risk of secondary infection caused by external agents. Accordingly, a survival rate and a recovery rate of the stromal vascular fraction with respect to the amount of adipose tissue are increased whereby less adipose tissue is required and thus the stromal vascular fraction is efficiently and easily separated from the adipose tissue.