Abstract: Provided are a primer and probe composition for cat blood type detection, a detection method, and use. The primer and probe composition for detecting the wild-types and the mutants of the above four sites is designed according to the single nucleotide polymorphism (SNP) mutation of genotyping of 179G>T, 268T>A, 364C>T and 1322delT four sites of a cat cytidine monophospho-N-acetylneuraminic acid (CMAH) gene; an amplification curve is plotted by utilizing a real-time fluorescence polymerase chain reaction (PCR) technology, and the recognition of bases of four SNP sites is achieved by analysis of the amplification curve, so as to determine cat blood types. The detection method of the present disclosure is carried out entirely in a closed space, thereby avoiding the problem of environmental aerosol pollution.

Abstract: Provided are a primer and probe composition for cat blood type detection, a detection method, and use. The primer and probe composition for detecting the wild-types and the mutants of the above four sites is designed according to the single nucleotide polymorphism (SNP) mutation of genotyping of 179G>T, 268T>A, 364C>T and 1322delT four sites of a cat cytidine monophospho-N-acetylneuraminic acid (CMAH) gene; an amplification curve is plotted by utilizing a real-time fluorescence polymerase chain reaction (PCR) technology, and the recognition of bases of four SNP sites is achieved by analysis of the amplification curve, so as to determine cat blood types. The detection method of the present disclosure is carried out entirely in a closed space, thereby avoiding the problem of environmental aerosol pollution.