Abstract: Provided in the present invention are a recombinant hemoglobin, a nucleic acid encoding the recombinant hemoglobin, a preparation method therefor, etc., so as to solve the current problems of complex processes, high production costs, low yields, etc. that are present in the process of improving the stability of the tetrameric structure of hemoglobin. The recombinant hemoglobin comprises four polypeptide chains, the polypeptide chains being two ? chains and two ? chains respectively, wherein the amino acid sequences of the four polypeptide chains are linked by means of a linker peptide, and the linker peptides are preferably used to sequentially link the amino acid sequences of the four polypeptide chains from the N terminus to the C terminus in any order.

Abstract: Provided is a polypeptide tag. The amino acid sequence of the polypeptide tag is Xaa1Xaa2Xaa3PHDYNXaa4Xaa5Xaa6 (SEQ ID NO: 37), wherein in the formula, Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, and Xaa6 are each independently an amino acid or none. The polypeptide tag is used for labeling a target protein. In a second aspect, provided is a polypeptide fusion protein, comprising the following two structures: (1) any polypeptide tag according to the first aspect, and (2) a target protein connected to the polypeptide tag. Also provided are an in vitro cell-free protein synthesis system and an application thereof in in vitro protein synthesis. By constructing the polypeptide tag and a target protein as a fusion protein, the expression of the labeled target protein can be effectively increased without removing the polypeptide tag.

Abstract: A display panel and a driving method thereof, and a display apparatus are provided. The display panel includes multiple pixel units. Each data line is electrically connected to a column of the multiple pixel units. A source driving module is electrically connected to multiple data lines and outputs data signals to the multiple data lines. Each scan line is electrically connected to a row of the multiple pixel units for inputting scan signals to the multiple pixel units. A gate driving module includes multiple gate driving units and multiple voltage division units. One gate driving unit is electrically connected to one voltage division unit and one scan line. The gate driving unit outputs a driving signal to the voltage division unit to form the scan signal. In a direction away from the source driving module, voltages of the scan signals in the multiple scan lines are sequentially increased.

Abstract: Provided are an ADH protein mutant and the use thereof. Compared with a wild-type ADH protein, the mutant is capable of (i) enhancing the expression purity, efficiency and yield of exogenous proteins in an in-vitro cell-free synthesis system; and/or (ii) reducing the binding ability of the mutant protein to Ni medium.

Abstract: The present disclosure discloses a composition, method and application thereof for improving the quality of in vitro maturation of oocytes and the pregnancy rate after in vitro produced embryos transfer. The present disclosure aims at the problems of low maturation rate and poor subsequent embryo development potential caused by metabolic disorders during in vitro maturation of oocytes, and provides a composition including two or more regulators in vitamin C, L-carnitine and thrombopoietin to regulate the metabolism of oocytes, thereby greatly improving the in vitro maturation rate of oocytes and the subsequent embryo development potential. The present disclosure not only improves the efficiency of in vitro embryo production of livestock, but also provides a reference for improving the efficiency of human assisted reproductive technology. The method is simple, safe and efficient, and has great application value and broad application prospects.

Abstract: The present disclosure provides a wireless power transfer system with a position detection function and a position adjustment method therefor, the system includes an energy transmission component and a signal transmission component, where the energy transmission component includes a power transmitting coil, a power receiving coil and a magnetically integrated resonant coil, and the signal transmission component includes a signal transmitting coil and a signal receiving coil; the power transmitting coil, the magnetically integrated resonant coil and the signal transmitting coil are sequentially stacked, and the power receiving coil and the signal receiving coil are stacked. During position detection, the magnetically integrated resonant coil and the signal receiving coil as detection coils detect position offsets along an X-axis and a Y-axis, respectively.

Abstract: A communication system, method, and device for a miniature intelligent sensor. The communication system includes a sensor, a power gateway, and a power Internet of things (IoT) server. The sensor is configured to obtain sensor data and send the sensor data to the power gateway according to a Bluetooth communication protocol. The power gateway is configured to receive the sensor data and perform protocol conversion on the sensor data to obtain protocol conversion data adapted to a message queuing telemetry transport (MQTT) protocol. The power gateway is configured to send the protocol conversion data to the power IoT server according to the MQTT protocol. The power IoT server is configured to receive the protocol conversion data and obtain server receiving data of the power IoT server according to the protocol conversion data.

Abstract: A light beam is imaged within a sample and images of the sample are generated based on light received from the sample in response to the light imaged within the sample. A wavefront modulating element modifies a wavefront of the received light and/or a wavefront of the light imaged within the sample. One or more known aberrations are introduced into at least one image of the sample and, based on at least two images of the sample, where the images include a raw image and at least one image that includes a known aberration, an aberration of a wavefront of light emitted from, and/or provided to, the sample is estimated. The wavefront modulating element is controlled to modulate the wavefront of light emitted from, and/or provided to, the sample, such that the estimated aberration of the wavefront of light emitted from, and/or provided to, the sample is reduced.

Abstract: The present disclosure belongs to the technical field of medicinal chemistry, and in particular relates to crystalline forms A, B, and C of a compound N-(3-fluorophenyl)-6-(6,7-dimethoxyquinolin-4-oxy)-3,4-dihydroquinoline-1(2H)-carboxamide methanesulfonate and preparation methods thereof. The three crystalline forms A, B, and C provided by the present disclosure can be prepared under various different conditions, a crystallization process has a good purification effect and has advantageous characteristics such as stable process and easy operation, the preparation methods for the crystalline forms are simple and has low cost, and different crystal forms of the compound N-(3-fluorophenyl)-6-(6,7-dimethoxyquinolin-4-oxy)-3,4-dihydroquinoline-1(2H)-carboxamide methanesulfonate with high purity, good solubility and good stability can be obtained.

Abstract: The present disclosure provides an additive for an electrolyte for a lithium battery, an electrolyte, and an electrochemical device. The additive includes a compound represented by the following formula I, wherein R1, R2, R3, and R4 are identical to or different from each other, and are independently selected from a group consisting of hydrogen, halogen, substituted or unsubstituted C1-C20 alkyl, and substituted or unsubstituted C6-C18 aryl, and a substituent of the substituted or unsubstituted C1-C20 alkyl or the substituted or unsubstituted C6-C18 aryl can be halogen, C1-C20 alkyl, or C6-C18 aryl; wherein denotes a single bond or a double bond; and wherein n is 1 or 2.

Abstract: Provided are a preparation method of a current collector assembly, a current collector assembly, a battery cell, and a battery pack. The preparation method of the current collector assembly includes: abutting the at least one of the connection plate or the protection plate having the protrusion against the plurality of tabs; generating a high-frequency vibration between the at least one of the connection plate or the protection plate having the protrusion and the plurality of tabs; and piercing the plurality of tabs sequentially by the protrusion through the high-frequency vibration. A vibration frequency of the high-frequency vibration ranges from 15 KHz to 40 KHz.

Abstract: The present disclosure discloses a method and system for designing a solution heat-treatment process of a single-crystal superalloy, and relates to the field of numerical simulation-material processing crossover techniques.

Abstract: A tandem DNA element capable of enhancing protein synthesis efficiency, in particular, the nucleic acid construct is formed by an IRES enhancer (such as ScBOI1, ScFLO8, ScNCE102, ScMSN1, KlFLO8, KlNCE102, KlMSN1, KlBOI1) derived from eukaryotic cells (such as yeast), a ? sequence, and a yeast-specific Kozak sequence in tandem. The use of the nucleic acid construct in a yeast-based in vitro biosynthesis system (such as a yeast-based in vitro protein synthesis system) can significantly improve protein synthesis efficiency.

Abstract: The present invention provides a polymer molecule, a monomeric structure and a polymeric structure comprising same, a related product, a preparation method, and uses. A polymer blocking the binding to a receptor is formed by the polymerization effect of the polymer molecule, thus effectively increasing the binding capability with respect to a virus. The polymer molecule is characterized in that: multiple binding-blocking molecular units used for blocking the binding between the virus and a cell receptor are polymerized into the polymeric structure.

Abstract: What provided is a method for regulating in vitro biosynthesis activity by knocking-out of a nuclease system, comprising: screening five nucleases among numerous nucleases, and performing down-regulation or knocking-out on one of the five nucleases (e.g., EXN53). The method can improve the stability of nucleic acid and the protein production efficiency of an in vitro protein synthesis system.

Abstract: A prediction method for water quality biotoxicity based on artificial intelligence neural network is provided, which relates to the field of water quality biotoxicity prediction technologies, and is used to solve a problem that water quality biotoxicity detection is limited to water quality organisms themselves. The prediction method uses a system including a water quality analysis unit configured to analyze simulated to-be-predicted waters to obtain variation values of water pollution, a fish analysis unit configured to analyze a fish situation of test fish to obtain fish abnormal values, a water quality determination unit configured to determine water quality of the simulated to-be-predicted waters to obtain an actual water quality level of the simulated to-be-predicted waters and send it to a data comparison unit, and the data comparison unit configured to compare data of water quality situation of the simulated to-be-predicted waters to obtain a prediction result of water quality biotoxicity.

Abstract: An in-vitro biosynthesis reaction apparatus and an in-vitro biosynthesis method. The in-vitro biosynthesis reaction apparatus comprises: a reaction body, which is used for containing a protein synthesis reaction liquid, so as to carry out an in-vitro biosynthesis reaction, wherein an opening part is arranged on the reaction body, and the opening part is provided with a feeding port used for enabling the synthesis reaction liquid to enter the reaction body and a discharging port allowing a reaction product to be discharged; and a partition part, which is used for incomplete separation of the internal space of the reaction body.

Abstract: To solve problems of frequent occurrence and great harm of membrane fouling during MBR wastewater treatment process, the invention proposes a membrane fouling intelligent early warning method to realize online and accurate early warning of membrane fouling. This early warning method achieves accurate prediction of water permeability by constructing soft-computing model based on recurrent fuzzy neural network. The intelligent early warning of membrane fouling is achieved by the comprehensive evaluation method, which solves the problem that membrane fouling is difficult to be early warning in the MBR wastewater treatment process, improves the pretreatment ability of membrane fouling, reduces the damage caused by membrane fouling, ensures the safe operation of MBR wastewater treatment process, and promotes efficient and stable operation of MBR wastewater treatment plant.

Abstract: Disclosed are a nickel-based superalloy formed by selective laser melting and a preparation method thereof. In the method, CrFeNb alloy powder is used as a grain refiner, and its element composition is within the composition range of a nickel-based superalloy powder to ensure that the prepared nickel-based superalloy has the same element composition with the original alloy; the grain size in the nickel-based superalloy could be refined by the addition of CrFeNb alloy powder, such that the anisotropic columnar grain structure in the alloy is transformed to equiaxed grain structure, thereby improving mechanical properties of the alloy.

Abstract: Disclosed are a nickel-based superalloy formed by selective laser melting and a preparation method thereof, belonging to the technical field of an additive manufacturing from metal powder. In the disclosure, CrFeNb alloy powder is used as a grain refiner, and its element composition is within the composition range of a nickel-based superalloy powder to ensure that the prepared nickel-based superalloy has the same element composition with the original alloy; the grain size in the nickel-based superalloy could be refined by the addition of CrFeNb alloy powder, such that the anisotropic columnar grain structure in the alloy is transformed to equiaxed grain structure, thereby improving mechanical properties of the alloy.