Abstract: The present invention relates to a nucleic acid amplification method and device, and a nucleic acid detection method and device. The nucleic acid amplification device comprises a reaction unit, an energy excitation unit, an operation unit, and an auxiliary cooling unit; the reaction unit comprises a target analyte to be detected, one or more solid-phase carriers, and a nucleic acid amplification reaction solution; each solid-phase carrier has a functionalized specific surface ligand to provide purification, separation, and nucleic acid amplification reaction of a target analyte to be detected; and energy output and an enabling/disabling time sequence of the energy excitation unit are controlled to generate a reaction temperature (thermal) cycle, so that an in-situ environment is formed around each solid-phase carrier during the cycle, and in-situ nucleic acid amplification reaction is performed in the in-situ environment to generate amplicons.

Abstract: A method for performing contactless ODEP for separation of CTCs is provided with the steps of obtaining patients' blood with rare cell suspected CTCs; adding at least one fluorescent antibody binding to CTCs into the blood; staining the blood; injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification; trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force; Separating the trapped CTCs from other non-CTCs cells; absorbing the trapped CTCs; and obtaining a high purity of CTCs. An apparatus for performing contactless ODEP for separation of CTCs is also provided.

Abstract: A method and a kit for detecting Mycobacterium tuberculosis are provided. The method includes a step of performing a nested qPCR assay to a specimen. The nested qPCR assay includes a first round of amplification using external primers and a second round of amplification using internal primers and a probe. The external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.

Abstract: A nucleic acid amplification system and a method thereof. An external energy source is used to drive nanoparticles with a regional thermal transfer to generate an energy response, so that the target nucleic acids on the nanoparticles are subjected to a polymerase chain reaction (PCR) or a nucleic acid isothermal amplification reaction, and the liquid in the reaction space is used for refrigeration to achieve rapid temperature regulation, such that the target nucleic acids accumulated on the surface of the nanoparticles are amplified. The concentration, capture and collection of the nanoparticles can be carried out by means of centrifugation, magnetic capture, lateral flow chromatography, etc. Subsequently, detection can be carried out by means of analyzing the fluorescence intensity and the color change of the layer after amplification.

Abstract: The present disclosure provides a method for screening, isolating and purifying analytes.

Abstract: The present invention discloses a nanoparticle control and detection system and operating method thereof. The present invention controls and detects the nanoparticles in the same device. The device comprises a first transparent electrode, a photoconductive layer, a spacer which is deposed on the edge of the photoconductive layer and a second transparent electrode. The aforementioned device controls and detects the nanoparticles by applying AC/DC bias and AC/DC light source to the transparent electrode.

Abstract: A cancer status prediction method and uses thereof, by analyzing specific cell information in blood samples and cancer clinical detection data with a cancer status analysis module to further perform cancer status prediction.

Abstract: A method for performing contactless ODEP for separation of CTCs is provided with the steps of obtaining patients' blood with rare cell suspected CTCs; adding at least one fluorescent antibody binding to CTCs into the blood; staining the blood; injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification; trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force; Separating the trapped CTCs from other non-CTCs cells; absorbing the trapped CTCs; and obtaining a high purity of CTCs. An apparatus for performing contactless ODEP for separation of CTCs is also provided.

Abstract: A apparatus for performing contactless ODEP for separation of CTCs comprises an ODEP device including a first conductive glass and a second conductive glass, the first conductive glass includes a transverse main channel and a longitudinal micro channel perpendicular to the main channel and joining the main channel at a cell separation zone; the first conductive glass includes a first hole and a second hole aligned with two ends of the main channel respectively, and a third hole aligned with one end of the micro channel that is distal to the cell separation zone; a sample receiving member disposed on and aligned with the first hole; an exhaust discharge member disposed on and aligned with the second hole; a target collection member disposed on and aligned with the third hole; and a controller including an optical projection device and an image fetch device.

Abstract: A method and a kit for detecting Mycobacterium tuberculosis are provided. The method includes a step of performing a nested qPCR assay to a specimen. The nested qPCR assay includes a first round of amplification using external primers and a second round of amplification using internal primers and a probe. The external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.

Abstract: A method of identifying properties of microorganisms using ODEP force includes obtaining a sample solution of microorganisms including microorganisms having predetermined properties; pre-processing the sample solution of microorganisms to obtain a sample solution of microorganisms to be tested having predetermined electrical properties; placing the sample solution of microorganisms to be tested on a channel of a chip member; activating a light image module having combinations of different strengths of ODEP force to analyze the sample solution of microorganisms to be tested on the channel in which microorganisms in the sample solution of microorganisms to be tested are configured to generate a predetermined distribution and a predetermined presentation due to the predetermined electrical properties; and identifying properties of the microorganisms based on the predetermined distribution and the predetermined presentation.

Abstract: An immunoassay includes forming first mixtures by reacting a combination reagent with serum of a subject; simultaneously forming second mixtures by reacting randomly selected platelet samples with the serum wherein in each mixture there are immunity compounds formed by combining the platelet antigens with predetermined antibodies in the serum, and other platelet antigens and other antibodies in the serum not forming the immunity compounds; preparing an interception device including receptacles and a filter net; placing each mixture in one receptacle; washing the mixtures wherein the mixtures forming the immunity compounds are intercepted by the filter net with others passing through; adding a signal sensing reagent to each receptacle; reacting the signal sensing reagent with the intercepted mixtures forming the immunity compounds to form final products; and performing a signal sensing to determine whether the final products contain anti-platelet antibodies and determine compatibility of cross matching of resp

Abstract: A method and a kit for detecting mycobacterium are provided. The method includes steps of: providing a sample; providing a pair of primers, which is selected from a group consisting of a sequence having about 45% to 100% similar to SEQ ID NO. 1, a sequence having about 60% to 100% similar to SEQ ID NO. 2, a sequence complementary thereof; performing a polymerase chain reaction by using the set of primers and the sample to obtain a product; and analyzing the product to detect the presence of the mycobacterium.

Abstract: A test sheet for occult blood includes a permeable substrate layer, a test layer, and an outer layer. The permeable substrate layer and the test layer are jointed side by side or stacked from top to bottom on the outer layer. The permeable substrate layer is configured to carry biologic sample, and the test layer includes test agent. The outer layer protects the user from the biologic sample. At least one fold line is formed on the front surface of the connection of the permeable substrate layer and the test layer, or alternatively, on the front surface of the permeable substrate layer. When the test sheet is folded along the fold line, the biologic sample permeates the permeable substrate layer and reacts with the test agent. If the reaction causes color change, the biologic sample is determined to contain occult blood.

Abstract: An apparatus for performing contactless ODEP for separation of CTCs comprises an ODEP device including a first conductive glass, a bio-compatible membrane, and a second conductive glass, the bio-compatible membrane includes a transverse main channel and a longitudinal micro channel perpendicular to the main channel and joining the main channel at a cell separation zone; the first conductive glass includes a first hole and a second hole aligned with two ends of the main channel respectively, and a third hole aligned with one end of the micro channel that is distal to the cell separation zone; a sample receiving member disposed on and aligned with the first hole; an exhaust discharge member disposed on and aligned with the second hole; a target collection member disposed on and aligned with the third hole; and a controller including an optical projection device and an image fetch device.

Abstract: Methods of assessing circulating tumor cells (CTCs) and methods of using the data obtained from assessing CTCs are provided. The methods employ negative selection and 3D cell culture techniques to isolate and culture CTCs. Those CTCs then can be used in different bioassays or evaluations, such as to determine tumor morphology, monitor tumor status, predict prognosis, provide treatment suggestion, and assess treatment effectiveness.

Abstract: The present invention discloses a nanoparticle control and detection system and operating method thereof. The present invention controls and detects the nanoparticles in the same device. The device comprises a first transparent electrode, a photoconductive layer, a spacer which is deposed on the edge of the photoconductive layer and a second transparent electrode. The aforementioned device controls and detects the nanoparticles by applying AC/DC bias and AC/DC light source to the transparent electrode.

Abstract: A method for detecting different properties in a microorganism population by ODEP force includes obtaining a microorganism sample solution having a plurality of microorganisms to be tested; pre-processing the microorganism sample solution to obtain a microorganism sample solution to be tested including the microorganisms having electrical properties differences; placing the microorganism sample solution to be tested in a channel of an ODEP device and activating an optical projection device to form at least one optical projection directed to the ODEP device; flowing the microorganism sample solution to be tested from one end of the channel to the other end thereof, and exerting an ODEP force on the optical projection device to generate a force having a direction different from a flowing direction of the microorganism sample solution to be tested; and detecting heterogeneity of the microorganisms based on strength differences of the ODEP force exerted on the respective microorganisms.

Abstract: The present disclosure provides a method for diagnosing cancer, assessing cancer prognosis, monitoring cancer, or assessing effectiveness of cancer treatment. The present disclosure also provides a method for diagnosing cancer, assessing cancer prognosis, monitoring cancer, or assessing effectiveness of cancer treatment by using an atypical circulating tumor cell.

Abstract: The present disclosure provides a method for screening, isolating and purifying analytes.