Abstract: Some aspects of this disclosure provide tissue constructs comprising a decellularized biomatrix and a neoplastic cell cultured within the biomatrix, as well as methods, reagents, and bioreactors for generating and using such tissue constructs. Tissue constructs as provided herein resemble clinically presenting tumors more closely than conventional in vitro and in vivo tumor models in various aspects, and can be used, for example, as tumor models for research and for the identification of anti-cancer agents.