Abstract: Provided is a microfluidic control chip, which includes a filter section having a filter to which anti-immunoglobulin antibodies, which are bound to endogenous antibodies in blood to thereby remove the endogenous antibodies, are immobilized, a first reaction section to which detection antibodies immobilized to fluorescent nano-particles are adsorbed, the detection antibodies being bound to proteins to be detected in blood which is introduced from the filter section with the endogenous antibodies removed therefrom, and a second reaction and detection section including capture antibodies immobilized thereto, binding the capture antibodies to the proteins, which are bound to the detection antibodies introduced from the first reaction section, and detecting a concentration of the proteins based on an intensity of fluorescent light. Thus, the microfluidic control chip can minimize interference of an immune response to maximize the immune response.

Abstract: Provided is a microfluidic control chip, which includes a filter section having a filter to which anti-immunoglobulin antibodies, which are bound to endogenous antibodies in blood to thereby remove the endogenous antibodies, are immobilized, a first reaction section to which detection antibodies immobilized to fluorescent nano-particles are adsorbed, the detection antibodies being bound to proteins to be detected in blood which is introduced from the filter section with the endogenous antibodies removed therefrom, and a second reaction and detection section including capture antibodies immobilized thereto, binding the capture antibodies to the proteins, which are bound to the detection antibodies introduced from the first reaction section, and detecting a concentration of the proteins based on an intensity of fluorescent light. Thus, the microfluidic control chip can minimize interference of an immune response to maximize the immune response.

Abstract: There is provided a cell culture technology for culturing an animal cell in a separate microstructure. The micro-scaled animal cell incubator includes a lower glass substrate having fine hot wires processed with a metal and formed in an upper surface thereof; a first PDMS film attached onto the lower glass substrate to form two or more liquid and solid storage spaces in a position corresponding to the fine hot wires of the lower glass substrate and gas flow channels coupled respectively to the liquid and solid storage spaces to allow generated gases to flow therethrough; a gas-permeable PDMS thin film attached onto the gas flow channels of the PDMS film to pass the generated gases therethrough; a second PDMS film attached onto the PDMS thin film and having a culture medium storage space for storing a culture medium; and an upper glass substrate attached onto the second PDMS film and having fine hot wires formed in a lower surface thereof, the fine hot wires being covered by the PDMS film.

Abstract: Provided are a method of chondrogenic differentiation from mesenchymal stem cells and a composition comprising chondrogenic cells differentiated using the method to treat diseases caused by cartilage damage. In the method, a centrifugal force is applied to human mesenchymal stem cells to be differentiated into chondrogenic cells. The chondrogenic differentiation may be achieved at moderate cost without using expensive cytokines or growth factors by periodically applying only a centrifugal force. According to the method of chondrogenic differentiation from mesenchymal stem cells in the present embodiment, chondrogenic cells may be also differentiated from human mesenchymal stem cells.

Abstract: There are provided a filter chip in which a filter is mounted on a microfluidic device as a hybrid form, and a method of manufacturing the filter chip. The method includes: forming a bottom structure where a groove for stably mounting a filter is formed; mounting the filter on the groove; forming a top structure forming a fluid inlet for injecting a fluid into the filter; and covering the top structure on a top area of the groove to attach to the bottom structure, wherein the groove and the filter have a shape becoming narrow from the fluid inlet to a fluid outlet in such a way that the fluid receives a rapid change of a capillary force while passing through the filter. Accordingly, blood plasma is capable of being separated at a higher speed by increasing the capillary force of the fluid outlet, thereby obtaining the blood plasma as large amount as possible.

Abstract: A method of manufacturing an ultrasonic welding-based microfluidic device, the method including: forming a bottom board having two welding stoppers formed right and left and having a certain height and a certain interval; forming a top board having two energy directors formed with an interval greater than the interval between the two welding stoppers; putting the top board on the bottom board to locate the energy directors at the outside of welding stoppers, respectively; and welding the top board to the bottom board by using ultrasonic welding, wherein a channel is formed between the two welding stoppers without additional energy directors. Accordingly, it is possible to prevent a phenomenon that a fluid irregularly flows due to an uneven surface formed on a side of the channel while the energy directors are melted.