Abstract: A sesame shattering resistance trait regulation gene Sihec3 and its application are provided. This gene is located on the third chromosome of sesame, is a recessive control gene, and fully explains the phenotypic variation of the shattering resistance trait. Genetic analysis of the shattering trait was conducted with F2 and F2:3 populations derived from a hybrid combination between a shattering resistance mutant M7 and normal shattering material. The research indicates that the seed shattering resistance trait in the tested population is a recessive trait controlled by a single gene. Based on the self-constructed sesame genome fine map and efficient gene mapping technology, the key gene Sihec3 and its allele SiHEC3 regulating the sesame shattering trait were cloned and compared. A corresponding gene marker named SSR1 for the sesame shattering resistance trait was dig. This invention provides a technical foundation for breeding new sesame varieties with shattering resistance trait.

Abstract: A gene SiFAD2-1 controlling the oleic acid content trait in sesame and its SNP marker SiSNPFAD2-1 are provided. This gene is located on chromosome 4 of sesame and belongs to an incomplete dominant control gene; compared with a wild-type allele Sifad2-1. The high oleic acid mutant gene SiFAD2-1 has a 100% explanation value for a mutant trait of sesame high oleic acid mutant HO995 (i.e., this gene controls a high oleic acid phenotype). The present application can provide a certain theoretical basis for studying the regulation mechanism of high oil acid in sesame and other crops, and also provide a material basis and genetic resources for developing molecular assisted breeding technology for sesame, breeding new varieties with high oleic acid in sesame and even other crops.

Abstract: A gene SiPT1 regulating a sesame plant architecture trait and a detection primer pair thereof are provided. This gene is located on 10th chromosome of sesame, belongs to a dominant control gene, has 100% explanation ratio for a branching trait; the gene has a length of 1318 bp, contains 4 exons and 3 introns, and its base sequence is shown in SEQ ID No. 1. Based on analysis and research of this gene, a good technical foundation can be laid for an early identification and rapid screening of sesame plant architecture in new varieties breeding; it has important technical significance for accelerating to breed new sesame varieties suitable for mechanized operations; can also provide a certain genetic foundation for the development of molecular assisted breeding technology and screening and breeding of new sesame varieties with different plant architectures.

Abstract: A sesame shattering resistance trait regulation gene Sihec3 and its application are provided. This gene is located on the third chromosome of sesame, is a recessive control gene, and fully explains the phenotypic variation of the shattering resistance trait. Genetic analysis of the shattering trait was conducted with F2 and F2:3 populations derived from a hybrid combination between a shattering resistance mutant M7 and normal shattering material. The research indicates that the seed shattering resistance trait in the tested population is a recessive trait controlled by a single gene. Based on the self-constructed sesame genome fine map and efficient gene mapping technology, the key gene Sihec3 and its allele SiHEC3 regulating the sesame shattering trait were cloned and compared. A corresponding gene marker named SSR1 for the sesame shattering resistance trait was dig. This invention provides a technical foundation for breeding new sesame varieties with shattering resistance trait.

Abstract: A Sidwf1 gene of Sesamum indicum, including two exons and an intron, is 1638 bp in total, and has a sequence represented by SEQ ID NO: 1. Also provided is a method for determining the internode length type in sesame samples, the method including: 1) extracting a genomic DNA of a sesame sample; 2) synthesizing three primers including SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R; amplifying the Sidwf1 gene or an allele SiDWF1 thereof with the genomic DNA of the sesame sample as a template, with a combination of SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R a combination of SiSNPdwf1 F1 and SiSNPdwf1 R, or a combination of SiSNPdwf1 F2 and SiSNPdwf1 R, as primers, thereby yielding a PCR product; and performing electrophoresis on the PCR product or sequencing the PCR product, and determining the phenotype of the sesame sample according to an electrophoresis or sequencing result.

Abstract: A Sidwf1 gene of Sesamum indicum, including two exons and an intron, is 1638 bp in total, and has a sequence represented by SEQ ID NO: 1. Also provided is a method for determining the internode length type in sesame samples, the method including: 1) extracting a genomic DNA of a sesame sample; 2) synthesizing three primers including SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R; amplifying the Sidwf1 gene or an allele SiDWF1 thereof with the genomic DNA of the sesame sample as a template, with a combination of SiSNPdwf1 F1, SiSNPdwf1 F2, and SiSNPdwf1 R a combination of SiSNPdwf1 F1 and SiSNPdwf1 R, or a combination of SiSNPdwf1 F2 and SiSNPdwf1 R, as primers, thereby yielding a PCR product; and performing electrophoresis on the PCR product or sequencing the PCR product, and determining the phenotype of the sesame sample according to an electrophoresis or sequencing result.