Abstract: Provided is a probe composition, including: a first probe including a first oligonucleotide strand and a first antibody, 5? end of the first oligonucleotide strand linked to the first antibody, and the first antibody having an activity of binding to a predetermined protein; a second probe including a second oligonucleotide strand and a second antibody, 5? end of the second oligonucleotide strand linked to the second antibody, the second antibody having an activity of binding to the predetermined protein, and at least a partial sequence at 3? end of the first oligonucleotide strand capable of reverse complementary pairing with at least a partial sequence at 3? end of the second oligonucleotide strand; and a third probe including a third oligonucleotide strand, at least a partial sequence at 5? end of the third oligonucleotide strand complementarily paired with a partial sequence at 3? end of the second oligonucleotide strand.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: A method including obtaining an optimization request at a coordinating engine. The method also can include triggering engines to process the optimization request. At least one of the engines divides the optimization request into subproblems. At least a portion of the engines solve the subproblems. Respective instances of at least one of the engines are triggered to handle respective ones of the subproblems. Each of the engines provides a dynamic algorithmic flow using modularized algorithmic solvers. The dynamic algorithmic flow is adjusted based on a respective input to each of the engines. The method additionally can include outputting, from the coordinating engine, one or more results in response to the optimization request, based on results for the subproblems generated by the engines. Other embodiments are described.

Abstract: A system including a processor and a non-transitory computer-readable media storing computing instructions that, when executed on the processor, cause the processor to perform certain operations: identifying a first transit time of a first load that is different from a second transit time of a carrier for the first load; tuning a load generation model using a slackness allowance function; generating, using the load generation model with the slackness allowance function engaged, multiple slackness time windows with corresponding slackness penalties for the first load; and determining a third transit time based on a slackness time window of the multiple slackness time windows and a corresponding slackness penalty for the first load. Other embodiments are disclosed.

Abstract: A system including a processor and a non-transitory computer-readable media storing computing instructions that, when executed on the processor, cause the processor to perform certain operations: obtaining a distribution of transit miles for inbound loads located within a distance of each facility of multiple facilities; creating, using a partition engine, multiple spatial partitions within the distance of each facility of the multiple facilities; determining, using the partition engine, a multi-partition distance by combining multiple spatial partitions with overlapping distances; determining shipment consolidation options for the inbound loads within the multi-partition distance of the multiple facilities; and generating a transit plan corresponding to the inbound loads located within the multi-partition distance, based on the shipment consolidation options. Other embodiments are described.

Abstract: A method including obtaining information about a batch of loads. The method also can include determining one or more respective alternative assignments that are feasible for each of the loads. The method additionally can include generating an assignment based on selecting a respective selected carrier for each of the loads from among the one or more respective alternative assignments for each of the loads. Generating the assignment can include using respective deviation scores when there are multiple primary carriers among the one or more respective alternative assignments. The method further can include modifying the assignment for a subset of the loads based on a flex rate. The method additionally can include outputting the assignment, as modified. Other embodiments are described.

Abstract: A system including a processor and a non-transitory computer-readable media storing computing instructions that, when executed on the processor, cause the processor to perform certain operations: splitting a set of candidate loads into subsets of candidate loads, wherein time windows of the subsets of candidate loads align with time ranges of a facility; iteratively reducing, without integer constraints, the subsets of candidate loads into a number of candidate loads; determining multiple candidate loads from the number of candidate loads that optimize an objective function with the integer constraints; and selecting at least one candidate load from the multiple candidate loads corresponding to a mode of transportation. Other embodiments are described.

Abstract: Provided are a luciferase mutant and the use thereof, and specifically provided are a copepod luciferase mutant and the use thereof. By means of performing protein directed evolution on Pxluc, a mutant which has more than 2-fold increased substrate specificity to ZS26/F-CTZ and a mutant which has more than 4-fold increased substrate specificity to ZS2/F-CTZ are obtained. The luciferase can be subjected to prokaryotic expression, the purification process is simple, and large-scale production is facilitated. The luciferase has similar luminescence to Gluc, is easy to detect, and has broad application prospects in the fields of basic scientific research, biological monitoring, biochemical diagnosis, etc.

Abstract: Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) includes an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) includes an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light.

Abstract: A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

Abstract: Provided are a direct current resistance (DCR) detection method for batteries, a system, a device, and a storage medium. Multiple discharging current pulses are applied during the constant current charging process of a battery to form a negative pulse charging mode. The discharging current can be added during the charging process to quickly remove the polarization effect of a battery cell generated during the charging process, thereby improving the charging acceptance capacity of the battery cell, shortening the charging time and increasing the charging efficiency. At the same time, a first battery voltage is obtained during the discharging current pulse time period, and a second battery voltage is obtained during the constant current charging time period after the discharging current pulse, thereby obtaining a DCR value of the battery during actual charging and use. The DCR of the battery cell can be detected simultaneously in the negative pulse charging mode.

Abstract: A method including obtaining an optimization request at a coordinating engine. The method also can include triggering engines to process the optimization request. At least one of the engines divides the optimization request into subproblems. At least a portion of the engines solve the subproblems. Respective instances of at least one of the engines are triggered to handle respective ones of the subproblems. Each of the engines provides a dynamic algorithmic flow using modularized algorithmic solvers. The dynamic algorithmic flow is adjusted based on a respective input to each of the engines. The method additionally can include outputting, from the coordinating engine, one or more results in response to the optimization request, based on results for the subproblems generated by the engines. Other embodiments are described.

Abstract: This application provides a charging control method, a charging control device, an electronic device, and a storage medium. The charging control method includes: obtaining fastest-charge data, where a charging time in the fastest-charge data is less than or equal to a minimum charging time for a same state-of-charge (SOC) increment section in historical charging processes; and performing charging control on a battery based on the fastest-charge data. By obtaining the fastest-charge data and performing charging control on the battery based on the fastest-charge data, the charging control method according to this application can ensure that the charging time is close to or equal to the minimum charging time in historical charging processes, thereby effectively increasing the charging speed.

Abstract: Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) includes an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) includes an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light.

Abstract: A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

Abstract: An article support device has a pair of support platforms arranged symmetrically and opposite to each other. Each of the support platforms includes a support frame, a support plate disposed on a top of the support frame and having a horizontal support top surface, and at least one positioning block installed on the horizontal support top surface. The horizontal support top surfaces of the support plates are located at a same height position. The article is supported on the horizontal support top surfaces and positioned between the positioning blocks on the support plates. An accommodation space is defined between the support plates and the support frames, and an automated guided vehicle moves into the accommodation space. An article carried on the article support device is loadable onto the automatic guided vehicle or the article carried on the automatic guided vehicle is unloadable onto the article support device.

Abstract: A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

Abstract: A system comprising one or more processors and one or more non-transitory computer-readable media storing computing instructions that, when executed on the one or more processors, cause the one or more processors to perform operations comprising: generating a schedule summary comprising one or more selectable portions of availability for drivers; receiving a selection of an unfilled portion of a respective schedule for a respective driver of the drivers adds the respective driver to a list of respective drivers to be scheduled on the schedule summary displayed on a GUI of an electronic device of a user; receiving one or more selections of the one or more selectable portions of availability of the schedule summary causes one or more changes to occur on the schedule summary displayed on the GUI of the electronic device of the user; and displaying one or more schedules for the drivers. Other embodiments are disclosed herein.

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing. FIG.

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.