Abstract: A continuous bioaerosol sampling device includes a sampling component, a liquid replenishing component, and a control component, where the sampling component includes a sampling cup, a sampling head, and a fan configured to drive the ambient air from the air-outlet passage of the sampling head to form an air flow spirally flowing along an axis of the sampling cup, allow the particulate matters to mix and react with the sampling solution in the sampling cup to form a sample solution, and further allow the remaining air flow to flow outside through the air-outlet passage of the sampling head; the liquid replenishing component is configured to supply the sampling solution from a liquid storage container to the sampling cup through a pump; and the control component is configured to control the pump and the fan according to signals sent from a liquid shortage sensor and a liquid level sensor.