Abstract: The present invention provides methods of producing single-stranded DNA (ssDNA) from a double-stranded DNA (dsDNA) template. The method includes selecting either the (+)-strand or the (?)-strand of a dsDNA template as a target ssDNA, wherein if the target ssDNA is the (+)-strand of the dsDNA template, a biotin label is added onto the reverse primer, and wherein if the target ssDNA is the (?)-strand of the dsDNA template, a biotin label is added onto the forward primer. Next PCR is performed to produce biotinylated dsDNA fragments. Once the PCR is terminated, the biotinylated dsDNA fragments are purified. Next the target ssDNA is separated from the biotinylated dsDNA by immobilizing the biotinylated dsDNA onto a surface and precipitating the target ssDNA.

Abstract: The present invention relates generally to PCR-based in vitro diagnostics and methods of treatment for SARS-CoV-2, the virus that causes COVID-19, and more specifically, to compositions and methods for the detection of wildtype and variants of SARS-CoV-2 in a patient specimen and methods of treating SARS-CoV-2 based on said detection.

Abstract: Methods for distinguishing between cotton cultivars of a specific species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the cultivar of a particular cotton species utilized in the textile or cotton material of interest.

Abstract: Methods for distinguishing between cotton species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the species of cotton utilized in the textile or cotton material of interest.

Abstract: This invention pertains to methods for authenticating an article comprising tagging the article with light emitting optical reporter particles, and more specifically tagging the articles with up-converting phosphor particles (UCP), linked to nucleic acids of detectable sequence.

Abstract: A method for authenticating and verifying a pharmaceutical item to be genuine is described. The method for authenticating a tablet comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to a tablet or capsule. The method also comprises collecting a sample of the tablet and verifying the tablet is genuine by detecting the particular nucleic acid material.

Abstract: A method for authenticating and verifying garment to be genuine is described. The method for authenticating a garment comprises applying a particular nucleic acid material/marker associated with a particular sequence of nucleic acid bases to a dye or paint and applying the marker to the garment. The method also comprises collecting a sample from the garment and verifying the garment is genuine by detecting the particular nucleic acid material on or within the garment.

Abstract: A method for authenticating and verifying an item to be genuine is described. The method for authenticating the item comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to ink within an ink cartridge or a toner compound within a toner housing. The method also comprises collecting a sample of either the ink or toner compound and verifying the ink or toner is genuine by detecting the particular nucleic acid material.

Abstract: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.

Abstract: A method to link a light emitting reporter to biomolecules with nucleotide oligomers is described. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particle generated by the methods of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.

Abstract: A method to link a light emitting reporter to biomolecules with nucleotide oligomers is described. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particle generated by the methods of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.

Abstract: Methods for distinguishing between cotton species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the species of cotton utilized in the textile or cotton material of interest.

Abstract: Methods for distinguishing between cotton cultivars of a specific species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the cultivar of a particular cotton species utilized in the textile or cotton material of interest.

Abstract: A method for authenticating and verifying a pharmaceutical item to be genuine is described. The method for authenticating a tablet comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to a tablet or capsule. The method also comprises collecting a sample of the tablet and verifying the tablet is genuine by detecting the particular nucleic acid material.

Abstract: A method for authenticating and verifying an item to be genuine is described. The method for authenticating the item comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to ink within an ink cartridge or a toner compound within a toner housing. The method also comprises collecting a sample of either the ink or toner compound and verifying the ink or toner is genuine by detecting the particular nucleic acid material.

Abstract: A method to link a light emitting reporter to biomolecules with nucleotide oligomers is described. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particle generated by the methods of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.

Abstract: This invention pertains to methods for authenticating an article comprising tagging the article with light emitting optical reporter particles, and more specifically tagging the articles with up-converting phosphor particles (UCP), linked to nucleic acids of detectable sequence.

Abstract: This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.

Abstract: A method for authenticating and verifying garment to be genuine is described. The method for authenticating a garment comprises applying a particular nucleic acid material/marker associated with a particular sequence of nucleic acid bases to a dye or paint and applying the marker to the garment. The method also comprises collecting a sample from the garment and verifying the garment is genuine by detecting the particular nucleic acid material on or within the garment.

Abstract: A method for authenticating a product where the product is composed of a plurality of components is described. The method for authenticating the product comprises providing a liquid compound, e.g. paint, that comprises at least one invisible market, e.g. nucleic acid, in which the compound is associated with a first entity. The method then proceeds to apply the compound to the surface of the product at the juncture of at least two components. The method also comprises associating the application of the compound to the at least two components with the first entity.