Abstract: Disclosed is a reagent kit and a nucleic acid extraction method. The kit is used for nucleic acid sample extraction. The kit comprises a hydrogen peroxide solution, and the molarity of the hydrogen peroxide solution is 0.1-2 mol/L. For certain nucleic acid samples, microbial cells included therein are wrapped by substances such as mucus or mucin and are difficult to release. After the hydrogen peroxide solution is added, substances in the microbial cells react with hydrogen peroxide to generate gas, and generated bubbles can destroy mucus or block-shaped samples, causing the samples to be completely liquefied, so as to release the microbial cells wrapped by the substances such as mucus or mucin, so that subsequent nucleic acid extraction steps can be smoothly carried out.

Abstract: The present disclosure provides an additive for PCR amplification, a multiplex PCR amplification kit, and use thereof, wherein the additive comprises benzalkonium bromide. The additive, when added to a simplex PCR system or a multiplex PCR system, can provide great promoting effects on the amplification efficiency and specificity of the simplex PCR amplification or multiplex PCR amplification, and thus possess great application prospects in simplex PCR or multiplex PCR.