Abstract: Disclosed are a method for measuring the inhibition of bacteria by an antibacterial drug, and a bacterial counting device and method thereof. The inhibition measurement method includes: adding a predetermined concentration of antibacterial drug to bacteria to be detected and setting as a mixture of bacteria and drug, while also setting the bacteria to be detected without the antibacterial drug as a positive control; when a first predetermined duration is reached from the time when the antibacterial drug is added, obtaining the current number of the bacteria in the mixture of bacteria and drug mixture of bacteria and drug and the current number of the bacteria in the positive control; determining that the antibacterial drug at the predetermined concentration inhibits or partially inhibits or does not inhibit the bacteria.