Abstract: The present invention provides a cochlear outer hair cell (OHC) regenerated by ectopic joint overexpression of Atoh1 and Ikzf2 and an application thereof. Specifically, the present invention provides an active ingredient combination capable of being used for regenerating a cochlear OHC, and a use thereof. The active ingredient combination comprises an Ikzf2 protein and an Atoh1 protein; and after the active ingredient combination is administered to a subject suffering from hearing impairment related to cochlear OHC degeneration and/or injury, hearing impairment can be alleviated by regenerating an OHC-like cell. A new method is provided for clinical treatment of hearing impairment.

Abstract: A method for changing filler pollutant accumulation of a constructed wetland belongs to the field of environmental protection engineering. A coupling device of a microbial fuel cell and a constructed wetland is constructed by using active carbon as a constructed wetland filler, and pond sewage enters into the constructed wetland from the top of the device in an intermittent mode. A titanium mesh is taken as an electron collector for packaging a cathode of the active carbon filler by using the characteristic that electrons are collected by the titanium mesh in a concentrated mode, after stable operation for a period of time, active carbon close to a water surface and active carbon close to the bottom of the titanium mesh are taken out for carrying out specific surface area and biomass measurement, and the accumulation distribution condition of filler pollutants inside the constructed wetland is analyzed.

Abstract: A method for changing filler pollutant accumulation of a constructed wetland belongs to the field of environmental protection engineering. A coupling device of a microbial fuel cell and a constructed wetland is constructed by using active carbon as a constructed wetland filler, and pond sewage enters into the constructed wetland from the top of the device in an intermittent mode. A titanium mesh is taken as an electron collector for packaging a cathode of the active carbon filler by using the characteristic that electrons are collected by the titanium mesh in a concentrated mode, after stable operation for a period of time, active carbon close to a water surface and active carbon close to the bottom of the titanium mesh are taken out for carrying out specific surface area and biomass measurement, and the accumulation distribution condition of filler pollutants inside the constructed wetland is analyzed.