Patents by Inventor Minoru Hiruma

Minoru Hiruma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay
    Patent number: 5260197
    Abstract: L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2):(1) Action and substrate specificityIt withdraws hydrogen from L-fucose and converts into L-fuconolactone and at the same time, reduces coenzyme NADP.sup.+ to NADPH.(2) pH and stable pH rangeWhen Tris-imidazole-sodium acetate buffer solution is used, the optimum pH is in a range of 9.0 to 10.0 and the stable pH range is between 8.0 and 10.5.The L-fucose dehydrogenase utilizing NADP.sup.+ as coenzyme can be obtained by culturing L-fucose dehydrogenase-producing strain belonging to the genus Pseudomonas in a medium and collecting formed L-fucose dehydrogenase from the culture.L-Fucose can be quantitatively determined by reacting a sample containing L-fucose with L-fucose dehydrogenase requiring NADP.sup.+ as coenzyme and assaying NADPH produced.
    Type: Grant
    Filed: September 14, 1989
    Date of Patent: November 9, 1993
    Assignee: Noda Institute for Scientific Research
    Inventors: Tatsuo Horiuchi, Minoru Hiruma, Toshiyuki Suzuki